BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5028

Title: Solution Structure of MCOTI-II, a Macrocyclic Trypsin Inhibitor

Authors: Felizmenio-Quimio, M.; Daly, N.; Craik, D.

Citation: Felizmenio-Quimio, M.; Daly, N.; Craik, D.. "Circular Proteins in Plants: Solution Structure of a Novel Macrocyclic Trypsin Inhibitor from Momordica cochinchinensis"  J. Biol. Chem. ., .-. (2001).

Assembly members:
MCoTI II, polymer, 34 residues, 3453 Da.

Natural source:   Common Name: spiny bitter cucumber   Taxonomy ID: 3674   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Momordica cochinchinensis

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
MCoTI II: SGSDGGVCPKILKKCRRDSD CPGACICRGNGYCG

Data sets:
Data typeCount
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MCoTI II1

Entities:

Entity 1, MCoTI II 34 residues - 3453 Da.

1   SERGLYSERASPGLYGLYVALCYSPROLYS
2   ILELEULYSLYSCYSARGARGASPSERASP
3   CYSPROGLYALACYSILECYSARGGLYASN
4   GLYTYRCYSGLY

Samples:

sample_1: MCoTI II 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 3.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
DQF-COSYsample_1not availablesample_cond_1
E-COSYsample_1not availablesample_cond_1

Software:

xwinnmr v2.6 - collection

XEASY v1.3.7 - data analysis

DYANA v1.5 - structure solution

X-PLOR v3.851 - structure solution

NMR spectrometers:

  • Bruker AVANCE 750 MHz

Related Database Links:

BMRB 5176 7361
PDB
SP P82409