BMRB Entry 50302

Title:
Human beta-2 microglobulin D76N variant
Deposition date:
2020-05-29
Original release date:
2020-07-13
Authors:
Smith, Hugh; Guthertz, Nicolas; Cawood, Emma; Maya-Martinez, Roberto; Breeze, Alexander; Radford, Sheena
Citation:

Citation: Smith, Hugh; Guthertz, Nicolas; Cawood, Emma; Maya-Martinez, Roberto; Breeze, Alexander; Radford, Sheena. "The role of the IT-state in D76N b2-microglobulin aggregation"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 100 residues, 11730.18 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pINK-b2m

Data sets:
Data typeCount
13C chemical shifts350
15N chemical shifts87
1H chemical shifts515

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Beta-2 microglobulin D76N1

Entities:

Entity 1, Beta-2 microglobulin D76N 100 residues - 11730.18 Da.

1   METILEGLNARGTHRPROLYSILEGLNVAL
2   TYRSERARGHISPROALAGLUASNGLYLYS
3   SERASNPHELEUASNCYSTYRVALSERGLY
4   PHEHISPROSERASPILEGLUVALASPLEU
5   LEULYSASNGLYGLUARGILEGLULYSVAL
6   GLUHISSERASPLEUSERPHESERLYSASP
7   TRPSERPHETYRLEULEUTYRTYRTHRGLU
8   PHETHRPROTHRGLULYSASNGLUTYRALA
9   CYSARGVALASNHISVALTHRLEUSERGLN
10   PROLYSILEVALLYSTRPASPARGASPMET

Samples:

sample_1: Beta-2 microglobulin D76N, [U-100% 13C; U-100% 15N], 430 ± 10 uM; sodium phosphate 25 mM; sodium chloride 83.3 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 148 mM; pH: 7.4; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(c)CH-TOCSYsample_1isotropicsample_conditions_1
3D (h)CCH-TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.5 - collection

NMRPipe vVersion 10.9 - processing

CcpNmr Analysis v2.4.2 - data analysis

NMR spectrometers:

  • Oxford Oxford 750 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks