BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5031

Title: Sequence-specific 1H, 13C and 15N resonance assignments of the N-terminal, 135-residue domain of KaiA, a clock protein from Synechococcus elongatus

Authors: Vakonakis, Ioannis; Risinger, Aaron; Latham, Michael; Williams, Stanly; Golden, Susan; LiWang, Andy

Citation: Vakonakis, Ioannis; Risinger, Aaron; Latham, Michael; Williams, Stanly; Golden, Susan; LiWang, Andy. "Letter to the Editor: Sequence-specific 1H, 13C and 15N resonance assignments of the N-terminal, 135-residue domain of KaiA, a clock protein from Synechococcus elongatus"  J. Biomol. NMR 21, 179-180 (2001).

Assembly members:
KaiA, polymer, 135 residues, 15066 Da.

Natural source:   Common Name: Synechococcus elongatus   Taxonomy ID: 32046   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Synechococcus elongatus

Experimental source:   Production method: recombinant technology   Host organism: coli

Entity Sequences (FASTA):
KaiA: MLSQIAICIWVESTAILQDC QRALSADRYQLQVCESGEML LEYAQTHRDQIDCLILVAAN PSFRAVVQQLCFEGVVVPAI VVGDRDSEDPDEPAKEQLYH SAELHLGIHQLEQLPYQVDA ALAEFLRLAPVETMA

Data sets:
Data typeCount
1H chemical shifts959
13C chemical shifts596
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KaiA N-terminus1

Entities:

Entity 1, KaiA N-terminus 135 residues - 15066 Da.

1   METLEUSERGLNILEALAILECYSILETRP
2   VALGLUSERTHRALAILELEUGLNASPCYS
3   GLNARGALALEUSERALAASPARGTYRGLN
4   LEUGLNVALCYSGLUSERGLYGLUMETLEU
5   LEUGLUTYRALAGLNTHRHISARGASPGLN
6   ILEASPCYSLEUILELEUVALALAALAASN
7   PROSERPHEARGALAVALVALGLNGLNLEU
8   CYSPHEGLUGLYVALVALVALPROALAILE
9   VALVALGLYASPARGASPSERGLUASPPRO
10   ASPGLUPROALALYSGLUGLNLEUTYRHIS
11   SERALAGLULEUHISLEUGLYILEHISGLN
12   LEUGLUGLNLEUPROTYRGLNVALASPALA
13   ALALEUALAGLUPHELEUARGLEUALAPRO
14   VALGLUTHRMETALA

Samples:

sample_KaiA-135: KaiA, [U-99% 13C; U-99% 15N], 2.9 – 3.4 mM

Ex-cond_1: pH: 6.4; temperature: 298 K; ionic strength: 0.22 M

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available

Software:

NMRPipe v1.8 rev 2001.030.21.27 - NMR data processing

PIPP v4.2.6 - Spectra visualization

STAPP v4.2.6 - Spin system creation, partial sequence specific assignments

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAA37101 BAD78522
GB AAM82684 ABB57248 AJD58238
REF WP_011242646 WP_011377921 YP_171042 YP_400235
SP Q79PF6