BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5033

Title: NMR Solution Structures of the Antifungal Heliomicin   PubMed: 11580275

Authors: Lamberty, M.; Caille, A.; Landon, C.; Tassin-Moindrot, S.; Hetru, C.; Bulet, P.; Vovelle, F.

Citation: Lamberty, M.; Caille, A.; Landon, C.; Tassin-Moindrot, S.; Hetru, C.; Bulet, P.; Vovelle, F.. "Solution Structures of the Antifungal Heliomicin and a Selected Variant with both Antibacterial and Antifungal Activities"  Biochemistry 40, 11995-12003 (2001).

Assembly members:
DEFENSIN HELIOMICIN, polymer, 44 residues, Formula weight is not available

Natural source:   Common Name: Tobacco budworm   Taxonomy ID: 7102   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Heliothis virescens

Experimental source:   Production method: recombinant technology   Host organism: Saccharomyces cerevisiae

Entity Sequences (FASTA):
DEFENSIN HELIOMICIN: DKLIGSCVWGAVNYTSDCNG ECKRRGYKGGHCGSFANVNC WCET

Data sets:
Data typeCount
1H chemical shifts275
coupling constants34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DEFENSIN HELIOMICIN1

Entities:

Entity 1, DEFENSIN HELIOMICIN 44 residues - Formula weight is not available

1   ASPLYSLEUILEGLYSERCYSVALTRPGLY
2   ALAVALASNTYRTHRSERASPCYSASNGLY
3   GLUCYSLYSARGARGGLYTYRLYSGLYGLY
4   HISCYSGLYSERPHEALAASNVALASNCYS
5   TRPCYSGLUTHR

Samples:

sample_1: DEFENSIN HELIOMICIN 4 mM; sodium acetate buffer 40 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 40 mM; pH: 4.3; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H DQF-COSYnot availablenot availablenot available
2D 1H TQF-COSYnot availablenot availablenot available
2D 1H TOCSYnot availablenot availablenot available
2D 1H NOESYnot availablenot availablenot available

Software:

UXNMR - collection

XEASY - data analysis

DYANA - refinement

X-PLOR - refinement

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
GB ACR78445 ADR51145 AGS47128
SP P81544