BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5036

Title: Solution Structure and Backbone Dynamics of the DNA-Binding Domain of Mouse Sox-5   PubMed: 11266597

Authors: Cary, P.; Read, C.; Davis, B.; Driscoll, P.; Crane-Robinson, C.

Citation: Cary, P.; Read, C.; Davis, B.; Driscoll, P.; Crane-Robinson, C.. "Solution Structure and Backbone Dynamics of the DNA-Binding Domain of Mouse Sox-5"  Protein Sci. 10, 83-98 (2001).

Assembly members:
TRANSCRIPTION FACTOR SOX-5, polymer, 81 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
TRANSCRIPTION FACTOR SOX-5: GSPHIKRPMNAFMVWAKDER RKILQAFPDMHNSNISKILG SRWKAMTNLEKQPYYEEQAR LSKQHLEKYPDYKYKPRPKR T

Data sets:
Data typeCount
1H chemical shifts480
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRANSCRIPTION FACTOR SOX-51

Entities:

Entity 1, TRANSCRIPTION FACTOR SOX-5 81 residues - Formula weight is not available

1   GLYSERPROHISILELYSARGPROMETASN
2   ALAPHEMETVALTRPALALYSASPGLUARG
3   ARGLYSILELEUGLNALAPHEPROASPMET
4   HISASNSERASNILESERLYSILELEUGLY
5   SERARGTRPLYSALAMETTHRASNLEUGLU
6   LYSGLNPROTYRTYRGLUGLUGLNALAARG
7   LEUSERLYSGLNHISLEUGLULYSTYRPRO
8   ASPTYRLYSTYRLYSPROARGPROLYSARG
9   THR

Samples:

sample_1: TRANSCRIPTION FACTOR SOX-5, [U-15N], 2 – 3 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; H2O 90%; D2O 10%

sample_2: TRANSCRIPTION FACTOR SOX-5 3 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; D2O 100%

sample_3: TRANSCRIPTION FACTOR SOX-5, [U-15N], 1 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; H2O 90%; D2O 10%

sample_4: TRANSCRIPTION FACTOR SOX-5 3 mM; potassium phosphate buffer 75 mM; DTT 0.5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.20; temperature: 298.00 K; ionic strength: 75 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H NOESYnot availablenot availablenot available
2D 15N-1H HMQC-Jnot availablenot availablenot available
3D 15N-1H NOESYnot availablenot availablenot available

Software:

X-PLOR v3.851 - refinement

FELIX v2.30 - data analysis

nmrview v3 - data analysis

InsightII v97.0 - data analysis

NMR spectrometers:

  • VARIAN UNITYplus 500 MHz

Related Database Links:

PDB
DBJ BAA32567 BAB85048 BAC05353 BAC16233 BAC16234
EMBL CAA09269 CAA46604 CAA46608 CAA46609 CAA46613
GB AAB49537 AAH29220 AAH35227 AAH47665 AAH60773
REF NP_001004385 NP_001028757 NP_001076940 NP_001107031 NP_001116382
SP B3DM43 P35710 P35711 P40647
TPG DAA29417