BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5038

Title: Assignments for human ubiquitin-conjugating enzyme 2b (HsUbc2b)   PubMed: 11885984

Authors: Miura, Takaaki; Klaus, Werner; Ross, Alfred; Guntert, Peter; Senn, Hans

Citation: Miura, Takaaki; Klaus, Werner; Ross, Alfred; Guntert, Peter; Senn, Hans. "The NMR Structure of the Class I Human Ubiquitin-conjugating Enzyme 2b"  J. Biomol. NMR 22, 89-92 (2002).

Assembly members:
ubiquitin-conjugating enzyme 2b, polymer, 152 residues, 17312 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ubiquitin-conjugating enzyme 2b: MSTPARRRLMRDFKRLQEDP PVGVSGAPSENNIMQWNAVI FGPEGTPFEDGTFKLVIEFS EEYPNKPPTVRFLSKMFHPN VYADGSICLDILQNRWSPTY DVSSILTSIQSLLDEPNPNS PANSQAAQLYQENKREYEKR VSAIVEQSWNDS

Data sets:
Data typeCount
1H chemical shifts1030
13C chemical shifts479
15N chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ubc2b1

Entities:

Entity 1, Ubc2b 152 residues - 17312 Da.

1   METSERTHRPROALAARGARGARGLEUMET
2   ARGASPPHELYSARGLEUGLNGLUASPPRO
3   PROVALGLYVALSERGLYALAPROSERGLU
4   ASNASNILEMETGLNTRPASNALAVALILE
5   PHEGLYPROGLUGLYTHRPROPHEGLUASP
6   GLYTHRPHELYSLEUVALILEGLUPHESER
7   GLUGLUTYRPROASNLYSPROPROTHRVAL
8   ARGPHELEUSERLYSMETPHEHISPROASN
9   VALTYRALAASPGLYSERILECYSLEUASP
10   ILELEUGLNASNARGTRPSERPROTHRTYR
11   ASPVALSERSERILELEUTHRSERILEGLN
12   SERLEULEUASPGLUPROASNPROASNSER
13   PROALAASNSERGLNALAALAGLNLEUTYR
14   GLNGLUASNLYSARGGLUTYRGLULYSARG
15   VALSERALAILEVALGLUGLNSERTRPASN
16   ASPSER

Samples:

sample_1: ubiquitin-conjugating enzyme 2b, [U-13C; U-15N], 1.0 mM; ammonium acetate, [U-2H], 75 mM; ammonium sulfate 100 mM

cond_set_1: pH: 6.7; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions

Software:

nmrPipe v1.7 -

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 17443
PDB
DBJ BAB26934 BAB27570 BAE28135 BAE40998 BAE88681
EMBL CAA37339 CAA65602 CAG28562 CAJ83014
GB AAA21087 AAA31492 AAA35982 AAB60669 AAC52884
PRF 2016220A
REF NP_001005124 NP_001032536 NP_001075765 NP_001085320 NP_001181362
SP P63146 P63147 P63148 P63149 Q32P99
TPG DAA27462