BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5041

Title: Solution structure and backbone dynamics of human DNA ligase IIIalpha BRCT domain   PubMed: 11683624

Authors: Krishnan, V.; Thornton, K.; Thelen, M.; Cosman, M.

Citation: Krishnan, V.; Thornton, K.; Thelen, M.; Cosman, M.. "Solution Structure and Backbone Dynamics of the Human DNA Ligase IIIalpha BRCT Domain"  Biochemistry 40, 13158-13166 (2001).

Assembly members:
DNA Ligase III, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
DNA Ligase III: GSADETLCQTKVLLDIFTGV RLYLPPSTPDFSRLRRYFVA FDGDLVQEFDMTSATHVLGS RDKNPAAQQVSPEWIWACIR KRRLVAPC

Data sets:
Data typeCount
1H chemical shifts493
13C chemical shifts310
15N chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA LIGASE III, unit I1
2DNA LIGASE III, unit II1

Entities:

Entity 1, DNA LIGASE III, unit I 88 residues - Formula weight is not available

1   GLYSERALAASPGLUTHRLEUCYSGLNTHR
2   LYSVALLEULEUASPILEPHETHRGLYVAL
3   ARGLEUTYRLEUPROPROSERTHRPROASP
4   PHESERARGLEUARGARGTYRPHEVALALA
5   PHEASPGLYASPLEUVALGLNGLUPHEASP
6   METTHRSERALATHRHISVALLEUGLYSER
7   ARGASPLYSASNPROALAALAGLNGLNVAL
8   SERPROGLUTRPILETRPALACYSILEARG
9   LYSARGARGLEUVALALAPROCYS

Samples:

sample_1: DNA Ligase III, [U-13C; U-15N], 1.0 mM; NaH2PO4 50 mM; NaCl 150 mM; DTT, [U-2H], 25 mM; H2O90 – 95%; D2O5 – 10%

sample_2: DNA Ligase III, [U-15N], 1.0 mM; NaH2PO4 50 mM; NaCl 150 mM; DTT, [U-2H], 25 mM; H2O90 – 95%; D2O5 – 10%

sample_cond_1: pH: 6.6; temperature: 288 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

FELIX v2.3 - processing

NMRPipe - data analysis

DYANA v1.5 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAH13269
EMBL CAA59230
GB AAH68005 ADP89974 ADP89976 ADP89978 ADP89980
REF NP_001252899 NP_039269 XP_003912656 XP_004041980 XP_005258027
SP P49916