BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5046

Title: NMR Structure of HCV IRES RNA Domain IIIC   PubMed: 15476802

Authors: Kaluarachchi, K.; Thiviyanathan, V; Rijnbrand, R.; Lemon, S.; Gorenstein, D.

Citation: Rijnbrand, R.; Thiviyanathan, V.; Kaluarachchi, K.; Lemon, S.; Gorenstein, D.. "Mutational and structural analysis of stem-loop IIIC of the hepatitis C virus and GB virus B internal ribosome entry sites"  J. Mol. Biol. 343, 805-817 (2004).

Assembly members:
Hepatitis C Internal Ribosome Entry Site, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 11102   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus not available

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
Hepatitis C Internal Ribosome Entry Site: GGGCGUGCCC

Data sets:
Data typeCount
1H chemical shifts89

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HEPATITIS C IRES RNA DOMAIN IIIC1

Entities:

Entity 1, HEPATITIS C IRES RNA DOMAIN IIIC 10 residues - Formula weight is not available

1   GGGCGUGCCC

Samples:

sample_1: Hepatitis C Internal Ribosome Entry Site1.0 – 2.0 mM; sodium phosphate buffer 10 mM; KCl 10 mM; EDTA 0.05 mM; H2O 90%; D2O 10%

sample_2: Hepatitis C Internal Ribosome Entry Site1.0 – 2.0 mM; sodium phosphate buffer 10 mM; KCl 10 mM; EDTA 0.05 mM; D2O 99.996%

sample_cond_1: pH: 6.8; temperature: 283 K; ionic strength: 10 mM; pressure: 1 atm

sample_cond_2: pH: 6.8; temperature: 298 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
31P Hetero-TOCSYnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available

Software:

X-PLOR v3.1 - refinement

MORASS v2.51 - refinement

NMR spectrometers:

  • Varian UNITYplus 600 MHz

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