BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5049

Title: Backbone 1H, 13C, and 15N data for the extracellular domain of human IFNAR2   PubMed: 11888273

Authors: Chill, Jordan; Nivasch, Rachel; Levy, Rina; Albeck, Shira; Schreiber, Gideon; Anglister, Jacob

Citation: Chill, Jordan; Nivasch, Rachel; Levy, Rina; Albeck, Shira; Schreiber, Gideon; Anglister, Jacob. "The Human Interferon Receptor: NMR-based Modeling, Mapping of the IFN-alpha2 Binding Site, and Observed Ligand-Induced Tightening"  Biochemistry 41, 3575-3585 (2002).

Assembly members:
Extracellular domain of subunit 2 of the Human receptor for type I interferons, polymer, 212 residues, 24850 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Extracellular domain of subunit 2 of the Human receptor for type I interferons: SYDSPDYTDESCTFKISLRN FRSILSWELKNHSIVPTHYT LLYTIMSKPEDLKVVKNCAN TTRSFCDLTDEWRSTHEAYV TVLEGFSGNTTLFSCSHNFW LAIDMSFEPPEFEIVGFTNH INVMVKFPSIVEEELQFDLS LVIEEQSEGIVKKHKPEIKG NMSGNFTYIIDKLIPNTNYC VSVYLEHSDEQAVIKSPLKC TLLPPGQESEFS

Data sets:
Data typeCount
1H chemical shifts582
13C chemical shifts573
15N chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IFNAR2-EC1

Entities:

Entity 1, IFNAR2-EC 212 residues - 24850 Da.

1   SERTYRASPSERPROASPTYRTHRASPGLU
2   SERCYSTHRPHELYSILESERLEUARGASN
3   PHEARGSERILELEUSERTRPGLULEULYS
4   ASNHISSERILEVALPROTHRHISTYRTHR
5   LEULEUTYRTHRILEMETSERLYSPROGLU
6   ASPLEULYSVALVALLYSASNCYSALAASN
7   THRTHRARGSERPHECYSASPLEUTHRASP
8   GLUTRPARGSERTHRHISGLUALATYRVAL
9   THRVALLEUGLUGLYPHESERGLYASNTHR
10   THRLEUPHESERCYSSERHISASNPHETRP
11   LEUALAILEASPMETSERPHEGLUPROPRO
12   GLUPHEGLUILEVALGLYPHETHRASNHIS
13   ILEASNVALMETVALLYSPHEPROSERILE
14   VALGLUGLUGLULEUGLNPHEASPLEUSER
15   LEUVALILEGLUGLUGLNSERGLUGLYILE
16   VALLYSLYSHISLYSPROGLUILELYSGLY
17   ASNMETSERGLYASNPHETHRTYRILEILE
18   ASPLYSLEUILEPROASNTHRASNTYRCYS
19   VALSERVALTYRLEUGLUHISSERASPGLU
20   GLNALAVALILELYSSERPROLEULYSCYS
21   THRLEULEUPROPROGLYGLNGLUSERGLU
22   PHESER

Samples:

sample_1: Extracellular domain of subunit 2 of the Human receptor for type I interferons, [U-97% 13C; U-97% 15N], 0.25 – 0.4 mM; NaN3 0.02%; NaCl0 – 1 mM; Tris, [U-2H], 25 mM

Cond-1: pH: 8.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
15N, 13C-4D separated NOESYnot availablenot availablenot available
13C, 13C-4D separated NOESYnot availablenot availablenot available
1H, 15N-HSQCnot availablenot availablenot available

Software:

AURELIA v2.8 - data analysis

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 16677
PDB
DBJ BAF85748 BAI46204
EMBL CAA54785 CAA61914 CAA61940 CAG46616 CDG37809
GB AAB46413 AAB46414 AAB46415 AAB46417 AAB46418
REF NP_000865 NP_001276054 NP_001276055 NP_001276057 NP_997467
SP P48551