BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5055

Title: Solution Structure of HI0257, a Bacterial Binding Protein   PubMed: 11551193

Authors: Parsons, L.; Eisenstein, E.; Orban, J.

Citation: Parsons, L.; Eisenstein, E.; Orban, J.. "Solution Structure of HI0257, a Bacterial Ribosome Binding Protein"  Biochemistry 40, 10979-10986 (2001).

Assembly members:
HI0257, polymer, 110 residues, 12472 Da.

Natural source:   Common Name: Haemophilus influenzae   Taxonomy ID: 727   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
HI0257: GSHMTLNITSKQMDITPAIR EHLEERLAKLGKWQTQLISP HFVLNKVPNGFSVEASIGTP LGNLLASATSDDMYKAINEV EEKLERQLNKLQHKSESRRA DERLKDSFEN

Data sets:
Data typeCount
1H chemical shifts664
13C chemical shifts397
15N chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HI02571

Entities:

Entity 1, HI0257 110 residues - 12472 Da.

1   GLYSERHISMETTHRLEUASNILETHRSER
2   LYSGLNMETASPILETHRPROALAILEARG
3   GLUHISLEUGLUGLUARGLEUALALYSLEU
4   GLYLYSTRPGLNTHRGLNLEUILESERPRO
5   HISPHEVALLEUASNLYSVALPROASNGLY
6   PHESERVALGLUALASERILEGLYTHRPRO
7   LEUGLYASNLEULEUALASERALATHRSER
8   ASPASPMETTYRLYSALAILEASNGLUVAL
9   GLUGLULYSLEUGLUARGGLNLEUASNLYS
10   LEUGLNHISLYSSERGLUSERARGARGALA
11   ASPGLUARGLEULYSASPSERPHEGLUASN

Samples:

sample_1: HI0257, [U-13C; U-15N], 1.3 mM; NaPO4 50 mM; NaCl 100 mM; dtt 3 mM

sample_cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCACONHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HBHACONHnot availablenot availablenot available
3D 15N TOCSY-HSQCnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

CNS v1.0 - refinement

NMRPipe - processing

Sparky - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

PDB
EMBL CBW28505 CBY80445 CBY86679
GB AAC21923 AAX87316 ABQ97834 ABQ99769 ADO80311
REF NP_438426 WP_005648863 WP_005653397 WP_005660635 WP_005689430
SP P71346