BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5059

Title: Chemical shift assignments for EC005 from E. coli   PubMed: 11854485

Authors: Yee, Adelinda; Chang, X.; Pineda_Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, Kalle; Kennedy, M.; Edwards, A.; Arrowsmith, Cheryl

Citation: Yee, Adelinda; Chang, X.; Pineda_Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, Kalle; Kennedy, M.; Edwards, A.; Arrowsmith, Cheryl. "An NMR Approach to Stuctural Proteomics"  Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).

Assembly members:
EC005, polymer, 97 residues, 10802.3 Da.

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
EC005: MGSSHHHHHHSSGLVPRGSH MKNIVPDYRLDMVGEPCPYP AVATLEAMPQLKKGEILEVV SDCPQSINNIPLDARNHGYT VLDIQQDGPTIRYLIQK

Data sets:
Data typeCount
1H chemical shifts350
13C chemical shifts126
15N chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EC0051

Entities:

Entity 1, EC005 97 residues - 10802.3 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METLYSASNILEVALPROASPTYRARGLEU
4   ASPMETVALGLYGLUPROCYSPROTYRPRO
5   ALAVALALATHRLEUGLUALAMETPROGLN
6   LEULYSLYSGLYGLUILELEUGLUVALVAL
7   SERASPCYSPROGLNSERILEASNASNILE
8   PROLEUASPALAARGASNHISGLYTYRTHR
9   VALLEUASPILEGLNGLNASPGLYPROTHR
10   ILEARGTYRLEUILEGLNLYS

Samples:

sample_1: EC005, [U-15N], 2.0 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM; NaN3 1 mM

sample_2: EC005, [U-15N; U-13C], 2.0 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM; NaN3 1 mM

sample_3: EC005 2.0 mM; phosphate buffer 50 mM; NaCl 0.15 M; DTT 1 mM; NaN3 1 mM

Ex-cond_1: pH: 7.5; temperature: 310 K; ionic strength: 0.3 M

Experiments:

NameSampleSample stateSample conditions
1H-15N TOCSYnot availablenot availableEx-cond_1
1H-15N NOESYnot availablenot availableEx-cond_1
1H-13C TOCSYnot availablenot availableEx-cond_1
1H-13C NOESYnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1

Software:

XWINNMR v2.1 - collection of data

GIFA v4.31 - data processing

XEASY v1.3.13 - data analysis

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAA15758 BAB36092 BAG77685 BAI26061 BAI30978
EMBL CAD05714 CAP76412 CAQ88691 CAQ98867 CAR03284
GB AAA71973 AAA82578 AAC74997 AAG56945 AAL20878
PIR AG0751
REF NP_288391 NP_310696 NP_416440 NP_456527 NP_460919
SP P0AA31 P0AA32 P0AA33 P0AA34 P0AA35