BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5065

Title: Quail Cysteine and Glycine-rich Protein, NMR, 15 Minimized Model Structures   PubMed: 11583159

Authors: Schuler, Wolfgang; Kloiber, Karin; Matt, Theresia; Bister, Klaus; Konrat, Robert

Citation: Schuler, Wolfgang; Kloiber, Karin; Matt, Theresia; Bister, Klaus; Konrat, Robert. "Application of Cross-correlated NMR Spin Relaxation to the Zinc-finger Protein CRP2(LIM2): Evidence for Collective Motions in LIM Domains"  Biochemistry 40, 9596-9604 (2001).

Assembly members:
CYSTEINE-RICH PROTEIN CRP2(LIM2), polymer, 113 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Japanese Quail   Taxonomy ID: 93934   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Coturnix japonica

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CYSTEINE-RICH PROTEIN CRP2(LIM2): MDRGERLGIKPESSPSPHRP TTNPNTSKFAQKFGGAEKCS RCGDSVYAAEKVIGAGKPWH KNCFRCAKCGKSLESTTLTE KEGEIYCKGCYAKNFGPKGF GYGQGAGALVHAQ

Data sets:
Data typeCount
13C chemical shifts231
15N chemical shifts56
1H chemical shifts336

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CYSTEINE-RICH PROTEIN1
2ZINC ION, I2
3ZINC ION, II2

Entities:

Entity 1, CYSTEINE-RICH PROTEIN 113 residues - Formula weight is not available

1   METASPARGGLYGLUARGLEUGLYILELYS
2   PROGLUSERSERPROSERPROHISARGPRO
3   THRTHRASNPROASNTHRSERLYSPHEALA
4   GLNLYSPHEGLYGLYALAGLULYSCYSSER
5   ARGCYSGLYASPSERVALTYRALAALAGLU
6   LYSVALILEGLYALAGLYLYSPROTRPHIS
7   LYSASNCYSPHEARGCYSALALYSCYSGLY
8   LYSSERLEUGLUSERTHRTHRLEUTHRGLU
9   LYSGLUGLYGLUILETYRCYSLYSGLYCYS
10   TYRALALYSASNPHEGLYPROLYSGLYPHE
11   GLYTYRGLYGLNGLYALAGLYALALEUVAL
12   HISALAGLN

Entity 2, ZINC ION, I - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: CYSTEINE-RICH PROTEIN CRP2(LIM2), [U-13C; U-15N], 1.0 – 2.0 mM; H2O 90%; D2O, [U-2H], 10%; potassium phosphate 20 mM; KCl 50 mM; dithiothreitol 0.5 mM

sample_2: CYSTEINE-RICH PROTEIN CRP2(LIM2), [U-15N], 1.0 – 2.0 mM; H2O 90%; D2O, [U-2H], 10%; potassium phosphate 20 mM; KCl 50 mM; dithiothreitol 0.5 mM

sample_cond_1: ionic strength: 70.5 mM; pH: 7.2; pressure: 1 atm; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablesample_cond_1
3D HNCAnot availablenot availablesample_cond_1
3D HNCACBnot availablenot availablesample_cond_1
3D HNCOnot availablenot availablesample_cond_1
3D CBCA(CO)NHnot availablenot availablesample_cond_1
3D HCCH-TOCSYnot availablenot availablesample_cond_1
3D CCH-TOCSY-NNHnot availablenot availablesample_cond_1
3D 13C-NOESY-HSQC (CA-centered)not availablenot availablesample_cond_1
3D 13C-NOESY-HSQC (methyl-centered)not availablenot availablesample_cond_1
3D 15N-NOESY-HSQCnot availablenot availablesample_cond_1

Software:

VNMR vv6.1 Rev.B - collection

NMRPipe vv1.8 Rev 2001.030.21.27 - processing

ANSIG vv3.3 - data analysis

CNS v1.0 - structure solution

X-PLOR v3.851 - refinement

NMR spectrometers:

  • VARIAN UNITYPLUS 500 MHz

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