BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5077

Title: 1H, 15N, 13C NMR Assignments of M156R Protein from myxoma virus, NESG target OP2   PubMed: 12367520

Authors: Ramelot, Theresa; Cort, John; Yee, Adelinda; Furong, L.; Goshe, M.; Edwards, Aled; Smith, R.; Arrowsmith, Cheryl; Dever, T.; Kennedy, Michael

Citation: Ramelot, Theresa; Cort, John; Yee, Adelinda; Furong, L.; Goshe, M.; Edwards, Aled; Smith, R.; Arrowsmith, Cheryl; Dever, T.; Kennedy, Michael. "Myxoma virus immunomodulatory protein M156R is a structural mimic of eukaryotic translation initiation factor eIF2a"  J. Mol. Biol. 322, 943-954 (2002).

Assembly members:
M156R, polymer, 102 residues, 11974 Da.

Natural source:   Common Name: Myxoma virus   Taxonomy ID: 10273   Superkingdom: Viruses   Kingdom: not available   Genus/species: Leporipoxvirus Poxvirus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
M156R: MTVIKPSSRPRPRKNKNIKV NTYRTSAMDLSPGSVHEGIV YFKDGIFKVRLLGYEGHECI LLDYLNYRQDTLDRLKERLV GRVIKTRVVRADGLYVDLRR FF

Data sets:
Data typeCount
1H chemical shifts563
13C chemical shifts374
15N chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1m156r1

Entities:

Entity 1, m156r 102 residues - 11974 Da.

1   METTHRVALILELYSPROSERSERARGPRO
2   ARGPROARGLYSASNLYSASNILELYSVAL
3   ASNTHRTYRARGTHRSERALAMETASPLEU
4   SERPROGLYSERVALHISGLUGLYILEVAL
5   TYRPHELYSASPGLYILEPHELYSVALARG
6   LEULEUGLYTYRGLUGLYHISGLUCYSILE
7   LEULEUASPTYRLEUASNTYRARGGLNASP
8   THRLEUASPARGLEULYSGLUARGLEUVAL
9   GLYARGVALILELYSTHRARGVALVALARG
10   ALAASPGLYLEUTYRVALASPLEUARGARG
11   PHEPHE

Samples:

sample_1: M156R, [U-15N; U-13C], 1 mM; phosphate buffer 25 mM; NaCl 450 mM

sample_2: M156R, [U-15N], 1 mM; phosphate buffer 25 mM; NaCl 450 mM

cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
15N-edited NOESY-HQSCnot availablenot availablenot available
HCC-TOCSY-NNHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CN-NOESY-HSQCnot availablenot availablenot available
CBCACOCAHAnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CCC-TOCSY-NNHnot availablenot availablenot available
HNHAnot availablenot availablenot available
4D CC-NOESYnot availablenot availablenot available

Software:

Felix v98 - processing data

XPLOR v3.84 - structure calculations

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz
  • Varian Inova 750 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
GB AAF15043 ACB28777 ACB28949 ADI75813 AFU77086
REF NP_051869