BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5083

Title: 1H and 15N chemical shift assignments for human epidermal-type fatty acid-binding protein (E-FABP)   PubMed: 12049637

Authors: Gutierrez-Gonzalez, Luis; Ludwig, Christian; Hohoff, Carsten; Rademacher, Martin; Hanhoff, Thorsten; Rueterjans, Heinz; Spener, Friedrich; Luecke, Christian

Citation: Gutierrez-Gonzalez, Luis; Ludwig, Christian; Hohoff, Carsten; Rademacher, Martin; Hanhoff, Thorsten; Rueterjans, Heinz; Spener, Friedrich; Luecke, Christian. "Solution Structure and Backbone Dynamics of Human Epidermal-Type Fatty Acid-Binding Protein (E-FABP)"  Biochem. J. 364, 725-737 (2002).

Assembly members:
Epidermal-type fatty acid binding protein, polymer, 135 residues, 15164 Da.
STE, non-polymer, 284.477 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Epidermal-type fatty acid binding protein: MATVQQLEGRWRLVDSKGFD EYMKELGVGIALRKMGAMAK PDCIITCDGKNLTIKTESTL KTTQFSCTLGEKFEETTADG RKTQTVCNFTDGALVQHQEW DGKESTITRKLKDGKLVVEC VMNNVTCTRIYEKVE

Data sets:
Data typeCount
1H chemical shifts966
15N chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E-FABP1
2stearate2

Entities:

Entity 1, E-FABP 135 residues - 15164 Da.

1   METALATHRVALGLNGLNLEUGLUGLYARG
2   TRPARGLEUVALASPSERLYSGLYPHEASP
3   GLUTYRMETLYSGLULEUGLYVALGLYILE
4   ALALEUARGLYSMETGLYALAMETALALYS
5   PROASPCYSILEILETHRCYSASPGLYLYS
6   ASNLEUTHRILELYSTHRGLUSERTHRLEU
7   LYSTHRTHRGLNPHESERCYSTHRLEUGLY
8   GLULYSPHEGLUGLUTHRTHRALAASPGLY
9   ARGLYSTHRGLNTHRVALCYSASNPHETHR
10   ASPGLYALALEUVALGLNHISGLNGLUTRP
11   ASPGLYLYSGLUSERTHRILETHRARGLYS
12   LEULYSASPGLYLYSLEUVALVALGLUCYS
13   VALMETASNASNVALTHRCYSTHRARGILE
14   TYRGLULYSVALGLU

Entity 2, stearate - C18 H36 O2 - 284.477 Da.

1   STE

Samples:

sample_1: Epidermal-type fatty acid binding protein1.5 – 2.0 mM; STEARIC ACID1.5 – 2.0 mM

sample_2: Epidermal-type fatty acid binding protein, [U-15N], 1.5 – 2.0 mM; STEARIC ACID1.5 – 2.0 mM

cond_1: pH: 5.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablecond_1
1H-1H NOESYnot availablenot availablecond_1
1H-15N HSQCnot availablenot availablecond_1
1H-15N HTQCnot availablenot availablecond_1
1H-15N TOCSY-HSQCnot availablenot availablecond_1
1H-15N NOESY-HSQCnot availablenot availablecond_1

Software:

XWINNMR v1.3 - data acquisition, data processing

AURELIA v2.5.9 - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 7107
PDB
DBJ BAG34797
GB AAA58467 AAH19385 AAH70303 AAP36117 AAX41860
REF NP_001247718 NP_001435 XP_002819257 XP_003831319 XP_003902954
SP Q01469