BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5084

Title: 1H and 15N Chemical Shift Assignments for the Complex of C-terminal Domain of Human Poly(A)-Binding Protein and 22-residue Fragment of Paip1   PubMed: 11287632

Authors: Kozlov, G.; Siddiqui, N.; Coillet-Matillon, S.; Ekiel, I.; Gehring, K.

Citation: Kozlov, G.; Trempe, J.; Khaleghpour, K.; Kahvejian, A.; Ekiel, I.; Gehring, K.. "Structure and function of the C-terminal PABC domain of human poly(A)-binding protein"  Proc. Natl. Acad. Sci. U. S. A. 98, 4409-4413 (2001).

Assembly members:
C-terminal domain of PABP, polymer, 98 residues, 10337 Da.
fragment of Paip1, polymer, 22 residues, 2426 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
C-terminal domain of PABP: GPLGSPLTASMLASAPPQEQ KQMLGERLFPLIQAMHPTLA GKITGMLLEIDNSELLHMLE SPESLRSKVDEAVAVLQAHQ AKEAAQKAVNSATGVPTV
fragment of Paip1: VLMSKLSVNAPEFYPSGYSS SY

Data sets:
Data typeCount
1H chemical shifts504
15N chemical shifts101
coupling constants18

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1polyadenylate-binding protein 1 (544-636)1
2polyadenylate-binding protein-interacting protein-1 (126-147)2

Entities:

Entity 1, polyadenylate-binding protein 1 (544-636) 98 residues - 10337 Da.

1   GLYPROLEUGLYSERPROLEUTHRALASER
2   METLEUALASERALAPROPROGLNGLUGLN
3   LYSGLNMETLEUGLYGLUARGLEUPHEPRO
4   LEUILEGLNALAMETHISPROTHRLEUALA
5   GLYLYSILETHRGLYMETLEULEUGLUILE
6   ASPASNSERGLULEULEUHISMETLEUGLU
7   SERPROGLUSERLEUARGSERLYSVALASP
8   GLUALAVALALAVALLEUGLNALAHISGLN
9   ALALYSGLUALAALAGLNLYSALAVALASN
10   SERALATHRGLYVALPROTHRVAL

Entity 2, polyadenylate-binding protein-interacting protein-1 (126-147) 22 residues - 2426 Da.

1   VALLEUMETSERLYSLEUSERVALASNALA
2   PROGLUPHETYRPROSERGLYTYRSERSER
3   SERTYR

Samples:

sample_1: C-terminal domain of PABP, [U-15N], 3 mM; fragment of Paip1, [U-15N], 3 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_2: C-terminal domain of PABP 3 mM; fragment of Paip1 3 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_3: C-terminal domain of PABP 3 mM; fragment of Paip1 3 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; D2O 100%

sample_4: C-terminal domain of PABP 3 mM; fragment of Paip1, [U-15N], 3 mM; phosphate buffer 50 mM; NaCl 0.1 M; NaN3 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.3; temperature: 303 K; ionic strength: 0.1 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

XWINNMR v2.1 - collection

GIFA v4.31 - processing

XEASY v1.3.13 - data analysis

ARIA v0.9 - structure solution

CNS v0.9 - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
EMBL CAB66854 CAB66854
GB AAC39697 AAH05295 AAH15937 ABM82036 ABW03359
REF NP_006442 NP_877590 NP_899152 XP_003811055 XP_003811056 NP_899152 NP_877590 NP_006442
SP Q9H074
SWISS-PROT Q9H074
GenBank ABW03359 ABM82036 AAH15937 AAH05295 AAC39697