BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5086

Title: Reduced recombinant Hydrogenobacter thermophilus cytochrome c-552 expressed in E. coli periplasm   PubMed: 11935350

Authors: Karan, Elizabeth; Russell, Brandy; Bren, Kara

Citation: Karan, Elizabeth; Russell, Brandy; Bren, Kara. "Characterization of Hydrogenobacter thermophilus Cytochromes c(552) Expressed in the Cytoplasm and Periplasm of Escherichia coli"  J. Biol. Inorg. Chem. 7, 260-272 (2002).

Assembly members:
cytochrome c-552, polymer, 80 residues, 9187 Da.
HEC, non-polymer, 618.503 Da.

Natural source:   Common Name: Hydrogenobacter thermophilus   Taxonomy ID: 940   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Hydrogenobacter thermophilus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
cytochrome c-552: NEQLAKQKGCMACHDLKAKK VGPAYADVAKKYAGRKDAVD YLAGKIKKGGSGVWGSVPMP PQNVTDAEAKQLAQWILSIK

Data sets:
Data typeCount
1H chemical shifts524

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ht p-cyt c-5521
2heme2

Entities:

Entity 1, Ht p-cyt c-552 80 residues - 9187 Da.

1   ASNGLUGLNLEUALALYSGLNLYSGLYCYS
2   METALACYSHISASPLEULYSALALYSLYS
3   VALGLYPROALATYRALAASPVALALALYS
4   LYSTYRALAGLYARGLYSASPALAVALASP
5   TYRLEUALAGLYLYSILELYSLYSGLYGLY
6   SERGLYVALTRPGLYSERVALPROMETPRO
7   PROGLNASNVALTHRASPALAGLUALALYS
8   GLNLEUALAGLNTRPILELEUSERILELYS

Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c-5522 – 3.5 mM; heme c2 – 3.5 mM; sodium acetate, [U-2H], 120 mM; sodium dithionite mM

condition_1: pH: 5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H TOCSYsample_1not availablecondition_1
1H NOESYsample_1not availablecondition_1

Software:

FELIX v97 -

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 10134 10135 17053 5087 5088 5089 6033
PDB
DBJ BAI69446
EMBL CAA40902
GB ADO45379
REF WP_012963626 YP_003432647 YP_005511672
SP P15452