BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5092

Title: 1H chemical shift assignments for the cytoplasmic N-terminus of KCNMB2   PubMed: 11517232

Authors: Bentrop, Detlef; Beyermann, Michael; Wissmann, Ralph; Fakler, Bernd

Citation: Bentrop, Detlef; Beyermann, Michael; Wissmann, Ralph; Fakler, Bernd. "NMR Structure of the "Ball-and-chain" Domain of KCNMB2, the beta 2-subunit of large Conductance Ca2+ and Voltage-activated Potassium Channels"  J. Biol. Chem. 276, 42116-41121 (2001).

Assembly members:
beta2 subunit of BK channels, polymer, 45 residues, 5367 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
beta2 subunit of BK channels: MFIWTSGRTSSSYRHDEKRN IYQKIRDHDLLDKRKTVTAL KAGED

Data sets:
Data typeCount
1H chemical shifts324

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminus of KCNMB21

Entities:

Entity 1, N-terminus of KCNMB2 45 residues - 5367 Da.

1   METPHEILETRPTHRSERGLYARGTHRSER
2   SERSERTYRARGHISASPGLULYSARGASN
3   ILETYRGLNLYSILEARGASPHISASPLEU
4   LEUASPLYSARGLYSTHRVALTHRALALEU
5   LYSALAGLYGLUASP

Samples:

sample_1: beta2 subunit of BK channels 2.0 mM

conditions_1: pH: 3.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
CLEAN-TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

XEASY -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB28216 BAG69598 BAG69600 BAG69602 BAG69604
GB AAD23380 AAF36562 AAH17825 AAH46227 AAH58957
REF NP_001073769 NP_001181373 NP_001265840 NP_005823 NP_082507
SP Q811Q0 Q9CZM9 Q9Y691
TPG DAA33284