BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5094

Title: Chemosensory Protein from Moth Mamestra brassicae. Expression and Secondary Structure from 1H and 15N NMR   PubMed: 11532009

Authors: Campanacci, V.; Mosbah, A.; Bornet, O.; Wechselberger, R.; Jacquin-Joly, E.; Cambillau, C.; Darbon, H.; Tegoni, M.

Citation: Campanacci, V.; Mosbah, A.; Bornet, O.; Wechselberger, R.; Jacquin-Joly, E.; Cambillau, C.; Darbon, H.; Tegoni, M.. "Chemosensory Protein from Moth Mamestra brassicae. Expression and Secondary Structure from 1H and 15N NMR"  Eur. J. Biochem. 268, 4731-4739 (2001).

Assembly members:
chemosensory protein, polymer, 112 residues, Formula weight is not available

Natural source:   Common Name: Mamestra brassicae   Taxonomy ID: 55057   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mamestra brassicae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
chemosensory protein: EDKYTDKYDNINLDEILANK RLLVAYVNCVMERGKCSPEG KELKEHLQDAIENGCKKCTE NQEKGAYRVIEHLIKNEIEI WRELTAKYDPTGNWRKKYED RAKAAGIVIPEE

Data sets:
Data typeCount
1H chemical shifts724
15N chemical shifts116
coupling constants96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chemosensory protein1

Entities:

Entity 1, chemosensory protein 112 residues - Formula weight is not available

1   GLUASPLYSTYRTHRASPLYSTYRASPASN
2   ILEASNLEUASPGLUILELEUALAASNLYS
3   ARGLEULEUVALALATYRVALASNCYSVAL
4   METGLUARGGLYLYSCYSSERPROGLUGLY
5   LYSGLULEULYSGLUHISLEUGLNASPALA
6   ILEGLUASNGLYCYSLYSLYSCYSTHRGLU
7   ASNGLNGLULYSGLYALATYRARGVALILE
8   GLUHISLEUILELYSASNGLUILEGLUILE
9   TRPARGGLULEUTHRALALYSTYRASPPRO
10   THRGLYASNTRPARGLYSLYSTYRGLUASP
11   ARGALALYSALAALAGLYILEVALILEPRO
12   GLUGLU

Samples:

sample_1: chemosensory protein 2 mM; sodium phosphate buffer 2 mM; H2O 90%; D2O 10%

sample_2: chemosensory protein, [U-15N], 0.5 mM; sodium phosphate buffer 0.5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.9; temperature: 296 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
clean-TOCSYnot availablenot availablenot available
3D 15N-1H-HSQC NOESYnot availablenot availablenot available
3D 15N-1H-HSQC TOCSYnot availablenot availablenot available
2D 15N-1H-HSQCnot availablenot availablenot available

Software:

XWINNMR v2.1 - processing

DIANA v2.8 - structure solution

DYANA v1.5 - structure solution

NMRPipe v1.8 - processing

CNS v1.0 - refinement

turbo frodo vMarch 2001 release a - data analysis

XEASY v1.3.13 - data analysis

Spscan v1.1 - processing

MOLMOL v2.6 - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
GB AAF19647 AAF19648 AAF19649 AAF19650 AAF19651