BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5096

Title: Solution Structure of a CCHH mutant of the ninth CCHC Zinc Finger of U-shaped   PubMed: 12110675

Authors: Kowalski, K.; Mackay, J.

Citation: Kowalski, K.; Liew, C.; Matthews, J.; Gell, D.; Crossley, M.; Mackay, J.. "Characterization of the Conserved Interaction between GATA and FOG Family Proteins"  J. Biol. Chem. 277, 35720-35729 (2002).

Assembly members:
U-shaped ninth zinc finger domain, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
U-shaped ninth zinc finger domain: GSAAEVMKKYCSTCDISFNY VKTYLAHKQFYHKNKP

Data sets:
Data typeCount
1H chemical shifts208
coupling constants24

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1U-shaped transcriptional cofactor1
2ZINC ION2

Entities:

Entity 1, U-shaped transcriptional cofactor 36 residues - Formula weight is not available

1   GLYSERALAALAGLUVALMETLYSLYSTYR
2   CYSSERTHRCYSASPILESERPHEASNTYR
3   VALLYSTHRTYRLEUALAHISLYSGLNPHE
4   TYRHISLYSASNLYSPRO

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: U-shaped ninth zinc finger domain 1.0 mM; ZnSO4 1.5 mM; TCEP 1.5 mM

sample_2: U-shaped ninth zinc finger domain, [U-95% 15N], 0.5 mM; ZnSO4 0.75 mM; TCEP 0.75 mM

sample_cond_1: ionic strength: 1.5 mM; pH: 5.0; pressure: 1 atm; temperature: 293 K

sample_cond_2: ionic strength: 0.75 mM; pH: 5.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

xwinnmr v2.6 - processing

DYANA v1.5 - structure solution

CNS v0.5 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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