BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5100

Title: 1H, 15N and 13C Assignments of FLIN2, an Intramolecular LMO2:ldb1 Complex   PubMed: 11824760

Authors: Matthews, Jacqueline; Visvader, Jane; Mackay, Joel

Citation: Matthews, Jacqueline; Visvader, Jane; Mackay, Joel. "1H, 15N and 13C Assignments of FLIN2, an Intramolecular LMO2:ldb1 Complex"  J. Biomol. NMR 21, 385-386 (2001).

Assembly members:
Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, polymer, 114 residues, 12589 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2: GSLLTCGGCQQNIGDRYFLK AIDQYWHEDCLSCDLCGCRL GEVGRRLYYKLGRKLCRRDY LRLGGSGGHMGSGGDVMVVG EPTLMGGEFGDEDERLITRL ENTQFDAANGIDDE

Data sets:
Data typeCount
13C chemical shifts344
15N chemical shifts115
1H chemical shifts1316

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FLIN21
2ZINC (II) ION, I2
3ZINC (II) ION, II2

Entities:

Entity 1, FLIN2 114 residues - 12589 Da.

1   GLYSERLEULEUTHRCYSGLYGLYCYSGLN
2   GLNASNILEGLYASPARGTYRPHELEULYS
3   ALAILEASPGLNTYRTRPHISGLUASPCYS
4   LEUSERCYSASPLEUCYSGLYCYSARGLEU
5   GLYGLUVALGLYARGARGLEUTYRTYRLYS
6   LEUGLYARGLYSLEUCYSARGARGASPTYR
7   LEUARGLEUGLYGLYSERGLYGLYHISMET
8   GLYSERGLYGLYASPVALMETVALVALGLY
9   GLUPROTHRLEUMETGLYGLYGLUPHEGLY
10   ASPGLUASPGLUARGLEUILETHRARGLEU
11   GLUASNTHRGLNPHEASPALAALAASNGLY
12   ILEASPASPGLU

Entity 2, ZINC (II) ION, I - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO20.3 – 0.5 mM; ZINC (II) ION0.6 – 1.0 mM

sample_2: Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, [U-95% 15N], 0.3 – 0.5 mM; ZINC (II) ION0.6 – 1.0 mM

sample_3: Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, [U-95% 13C; U-90% 15N], 0.3 – 0.5 mM; ZINC (II) ION0.6 – 1.0 mM

cond_1: ionic strength: 0.07 M; pH: 7.0; temperature: 298 K

cond_2: ionic strength: 0.07 M; pH: 5.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablenot available
1H-1H COSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
CC(CO)NH TOCSYnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNHAnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • BRUKER DMX 600 MHz

Related Database Links:

PDB
REF XP_001380687