BMRB Entry 5117

Title:
Structural and Dynamic Differences of Rhodostomin, an RGD-containing Disintegrin, and its D51E mutant
Deposition date:
2001-08-20
Original release date:
2003-04-09
Authors:
Chuang, Woei-Jer
Citation:

Citation: Guo, RT; Chou, LJ; Chen, YC; Chen, CY; Pari, K; Jen, CJ; Lo, SJ; Huang, SL; Lee, CY; Chang, TW; Chuang, Woei-Jer. "Structural and Dynamic Differences of Rhodostomin, an RGD-containing Disintegrin, and its D51E mutant"  Proteins: Struct., Funct., Genet. 43, 499-508 (2001).
PubMed: 11340665

Assembly members:

Assembly members:
Rhodostomin, polymer, 76 residues, 8417 Da.

Natural source:

Natural source:   Common Name: Snake   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Calloslasma rhodostoma

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts380
15N chemical shifts68

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rhodostomin1

Entities:

Entity 1, Rhodostomin 76 residues - 8417 Da.

1   GLUPHEHISHISHISHISHISHISGLYLYS
2   GLUCYSASPCYSSERSERPROGLUASNPRO
3   CYSCYSASPALAALATHRCYSLYSLEUARG
4   PROGLYALAGLNCYSGLYGLUGLYLEUCYS
5   CYSGLUGLNCYSLYSPHESERARGALAGLY
6   LYSILECYSARGILEPROARGGLYASPMET
7   PROASPASPARGCYSTHRGLYGLNSERALA
8   ASPCYSPROARGTYRHIS

Samples:

sample_1: Rhodostomin 3.0 mM

sample_2: Rhodostomin, [U-98% 15N], 2.5 mM

condition: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H DQF-COSYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available

Software:

xwinnmr v2.6 - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17963 2856 7399 7400
PDB
GB AAA57568

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks