BMRB Entry 5122

Title:
In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp. PCC6803 DnaB Mini-intein
Deposition date:
2001-08-23
Original release date:
2002-04-08
Authors:
Williams, Neal; Prosselkov, Pavel; Liepinsh, Edvards; Line, Inara; Sharipo, Anatoly; Littler, Dene; Curmi, Paul; Otting, Gottfried; Dixon, Nicholas
Citation:

Citation: Williams, Neal; Prosselkov, Pavel; Liepinsh, Edvards; Line, Inara; Sharipo, Anatoly; Littler, Dene; Curmi, Paul; Otting, Gottfried; Dixon, Nicholas. "In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp. PCC6803 DnaB Mini-intein"  J. Biol. Chem. 277, 7790-7798 (2002).
PubMed: 11742000

Assembly members:

Assembly members:
N-terminal Domain of E. coli DnaB Helicase, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts122
1H chemical shifts835

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cz-DnaB-N(24-136)1

Entities:

Entity 1, cz-DnaB-N(24-136) 122 residues - Formula weight is not available

1   LYSVALPROPROHISSERILEGLUALAGLU
2   GLNSERVALLEUGLYGLYLEUMETLEUASP
3   ASNGLUARGTRPASPASPVALALAGLUARG
4   VALVALALAASPASPPHETYRTHRARGPRO
5   HISARGHISILEPHETHRGLUMETALAARG
6   LEUGLNGLUSERGLYSERPROILEASPLEU
7   ILETHRLEUALAGLUSERLEUGLUARGGLN
8   GLYGLNLEUASPSERVALGLYGLYPHEALA
9   TYRLEUALAGLULEUSERLYSASNTHRPRO
10   SERALAALAASNILESERALATYRALAASP
11   ILEVALARGGLUARGALAVALVALARGGLU
12   METILESERTHRARGGLUSERGLYSERILE
13   GLUPHE

Samples:

sample_1: N-terminal Domain of E. coli DnaB Helicase 2.1 mM

sample_2: N-terminal Domain of E. coli DnaB Helicase, [U-95% 15N], 2.1 mM

ex-cond_1: ionic strength: 0.1 M; pH*: 7.0; temperature: 304 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
COSYnot availablenot availablenot available

Software:

Prosa v3.6 - spectra processing

XEASY v1.2 - peak analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

BMRB 4075 4297 5121
PDB
DBJ BAB38457 BAE78054 BAG66812
GenBank AAA23689 AAA23690 AAC43146 AAC77022 AAG59250
REF AP_004553 NP_290685 NP_313061 NP_418476 NP_709868
SWISS-PROT P0ACB0 P0ACB1 Q8FB22 Q8X5V3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks