BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5122

Title: In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp. PCC6803 DnaB Mini-intein   PubMed: 11742000

Authors: Williams, Neal; Prosselkov, Pavel; Liepinsh, Edvards; Line, Inara; Sharipo, Anatoly; Littler, Dene; Curmi, Paul; Otting, Gottfried; Dixon, Nicholas

Citation: Williams, Neal; Prosselkov, Pavel; Liepinsh, Edvards; Line, Inara; Sharipo, Anatoly; Littler, Dene; Curmi, Paul; Otting, Gottfried; Dixon, Nicholas. "In vivo Protein Cyclization Promoted by a Circularly Permuted Synechocystis sp. PCC6803 DnaB Mini-intein"  J. Biol. Chem. 277, 7790-7798 (2002).

Assembly members:
N-terminal Domain of E. coli DnaB Helicase, polymer, 122 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
N-terminal Domain of E. coli DnaB Helicase: KVPPHSIEAEQSVLGGLMLD NERWDDVAERVVADDFYTRP HRHIFTEMARLQESGSPIDL ITLAESLERQGQLDSVGGFA YLAELSKNTPSAANISAYAD IVRERAVVREMISTRESGSI EF

Data sets:
Data typeCount
1H chemical shifts835
15N chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cz-DnaB-N(24-136)1

Entities:

Entity 1, cz-DnaB-N(24-136) 122 residues - Formula weight is not available

1   LYSVALPROPROHISSERILEGLUALAGLU
2   GLNSERVALLEUGLYGLYLEUMETLEUASP
3   ASNGLUARGTRPASPASPVALALAGLUARG
4   VALVALALAASPASPPHETYRTHRARGPRO
5   HISARGHISILEPHETHRGLUMETALAARG
6   LEUGLNGLUSERGLYSERPROILEASPLEU
7   ILETHRLEUALAGLUSERLEUGLUARGGLN
8   GLYGLNLEUASPSERVALGLYGLYPHEALA
9   TYRLEUALAGLULEUSERLYSASNTHRPRO
10   SERALAALAASNILESERALATYRALAASP
11   ILEVALARGGLUARGALAVALVALARGGLU
12   METILESERTHRARGGLUSERGLYSERILE
13   GLUPHE

Samples:

sample_1: N-terminal Domain of E. coli DnaB Helicase 2.1 mM

sample_2: N-terminal Domain of E. coli DnaB Helicase, [U-95% 15N], 2.1 mM

ex-cond_1: pH*: 7.0; temperature: 304 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
COSYnot availablenot availablenot available

Software:

Prosa v3.6 - spectra processing

XEASY v1.2 - peak analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

BMRB 4075 4297 5121
PDB
DBJ BAB38457 BAE78054 BAG66812 BAG79869 BAH61192
EMBL CAP78509 CAQ34401 CAQ91563 CAR01031 CAR05689
GB AAA23689 AAA23690 AAC43146 AAC77022 AAG59250
REF NP_290685 NP_313061 NP_418476 NP_709868 NP_756878
SP P0ACB0 P0ACB1 Q8FB22 Q8X5V3