BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5141

Title: Sequence-specific resonance assignments of the N-terminal, 105-residue KaiC-interacting domain of SasA, a protein necessary for a robust circadian rhythm in Synechococcus elongatus

Authors: Klewer, Douglas; Williams, Stanly; Golden, Susan; LiWang, Andy

Citation: Klewer, Douglas; Williams, Stanly; Golden, Susan; LiWang, Andy. "Letter to the Editor: Sequence-specific resonance assignments of the N-terminal, 105-residue KaiC-interacting domain of SasA, a protein necessary for a robust circadian rhythm in Synechococcus elongatus"  J. Biomol. NMR 24, 77-78 (2002).

Assembly members:
SasA, polymer, 105 residues, 11476.3 Da.

Natural source:   Common Name: Synechoccus elongatus   Taxonomy ID: not available   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Synechoccus elongatus

Experimental source:   Production method: recombinant technology   Host organism: coli

Entity Sequences (FASTA):
SasA: GSSLSPQALAQPLLLQLFVD TRPLSQHIVQRVKNILAAVE ATVPISLQVINVADQPQLVE YYRLVVTPALVKIGPGSRQV LSGIDLTDQLANQLPQWLVQ QEGIF

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts104
1H chemical shifts683

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SasA N-terminus1

Entities:

Entity 1, SasA N-terminus 105 residues - 11476.3 Da.

1   GLYSERSERLEUSERPROGLNALALEUALA
2   GLNPROLEULEULEUGLNLEUPHEVALASP
3   THRARGPROLEUSERGLNHISILEVALGLN
4   ARGVALLYSASNILELEUALAALAVALGLU
5   ALATHRVALPROILESERLEUGLNVALILE
6   ASNVALALAASPGLNPROGLNLEUVALGLU
7   TYRTYRARGLEUVALVALTHRPROALALEU
8   VALLYSILEGLYPROGLYSERARGGLNVAL
9   LEUSERGLYILEASPLEUTHRASPGLNLEU
10   ALAASNGLNLEUPROGLNTRPLEUVALGLN
11   GLNGLUGLYILEPHE

Samples:

sample_SasA-105: SasA, [U-99% 13C; U-99% 15N], 0.6 mM

condition_1: ionic strength: 0.22 M; pH: 7.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
H(C)CH-COSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available

Software:

NMRPipe v1.8 rev 2001.030.21.27 - NMR data processing

PIPP v4.2.6 - Spectra visualization

STAPP v4.2.6 - partial sequence specific assignments, spin system creation

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAA03145 BAD80168
GB ABB58144 AJD57380
REF WP_011244288 WP_011378322 WP_039755926 YP_172688 YP_401131
SP Q06904