BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5145

Title: NMR Structure of the human Doppel Protein   PubMed: 12595265

Authors: Luhrs, Thorsten; Riek, Roland; Guntert, Peter; Wuthrich, Kurt

Citation: Luhrs, Thorsten; Riek, Roland; Guntert, Peter; Wuthrich, Kurt. "NMR Structure of the Human Doppel Protein"  J. Mol. Biol. 326, 1549-1557 (2003).

Assembly members:
Doppel Protein, polymer, 131 residues, 14967.7 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Doppel Protein: GSVQTRGIKHRIKWNRKALP STAQITEAQVAENRPGAFIK QGRKLDIDFGAEGNRYYEAN YWQFPDGIHYNGCSEANVTK EAFVTGCINATQAANQGEFQ KPDNKLHQQVLWRLVQELCS LKHCEFWLERG

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts406
15N chemical shifts150
1H chemical shifts898

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human Doppel(24-152)1

Entities:

Entity 1, human Doppel(24-152) 131 residues - 14967.7 Da.

1   GLYSERVALGLNTHRARGGLYILELYSHIS
2   ARGILELYSTRPASNARGLYSALALEUPRO
3   SERTHRALAGLNILETHRGLUALAGLNVAL
4   ALAGLUASNARGPROGLYALAPHEILELYS
5   GLNGLYARGLYSLEUASPILEASPPHEGLY
6   ALAGLUGLYASNARGTYRTYRGLUALAASN
7   TYRTRPGLNPHEPROASPGLYILEHISTYR
8   ASNGLYCYSSERGLUALAASNVALTHRLYS
9   GLUALAPHEVALTHRGLYCYSILEASNALA
10   THRGLNALAALAASNGLNGLYGLUPHEGLN
11   LYSPROASPASNLYSLEUHISGLNGLNVAL
12   LEUTRPARGLEUVALGLNGLULEUCYSSER
13   LEULYSHISCYSGLUPHETRPLEUGLUARG
14   GLY

Samples:

sample_1: Doppel Protein, [U-15N; U-13C], 1.5 mM

sample_2: Doppel Protein 2.0 mM

cond_1: ionic strength: 0.010 M; pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CACBnot availablenot availablenot available
1H-15N 3D-TOCSYnot availablenot availablenot available
HCCH COSYnot availablenot availablenot available
1H-15N 3D-NOESYnot availablenot availablenot available
1H-13C 3D-NOESYnot availablenot availablenot available
1H-1H 2D-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAG36200
GB AAF02424 AAG43448 AAG43449 AAH43644 AAQ89344
REF NP_036541
SP Q9UKY0