BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5152

Title: Designed Protein G Core Variants Fold to Native-like Structures: Sequence Selection by ORBIT Tolerates Variation in Backbone Specification   PubMed: 11266631

Authors: Ross, S.; Sarisky, C.; Su, A.; Mayo, S.

Citation: Ross, S.; Sarisky, C.; Su, A.; Mayo, S.. "Designed Protein G Core Variants Fold to Native-like Structures: Sequence Selection by ORBIT Tolerates Variation in Backbone Specification"  Protein Sci. 10, 450-454 (2001).

Assembly members:
IMMUNOGLOBULIN G BINDING PROTEIN G, polymer, 56 residues, Formula weight is not available

Natural source:   Common Name: Streptococcus sp.   Taxonomy ID: 1306   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptococcus sp.

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
IMMUNOGLOBULIN G BINDING PROTEIN G: TTFKLIINGKTLKGETTTEA VDAATAEKVLKQYINDNGID GEWTYDDATKTWTVTE

Data sets:
Data typeCount
1H chemical shifts371

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1immunoglobulin G binding protein G1

Entities:

Entity 1, immunoglobulin G binding protein G 56 residues - Formula weight is not available

1   THRTHRPHELYSLEUILEILEASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALLEU
4   LYSGLNTYRILEASNASPASNGLYILEASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRTRPTHRVALTHRGLU

Samples:

sample_1: IMMUNOGLOBULIN G BINDING PROTEIN G 1 mM; sodium phosphate 50 mM; H2O 90%; D2O 10%

sample_2: IMMUNOGLOBULIN G BINDING PROTEIN G 1 mM; sodium phosphate 50 mM; D2O 100%

sample_cond_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available

Software:

VNMR v5.2f - collection

VNMR v5.3a - collection

VNMR v6.1b - collection

NMRCompass v2.5 - processing

ANSIG v3.3 - processing

X-PLOR v3.1 - refinement, structure solution

NMR spectrometers:

  • Varian UNITYplus 600 MHz

Related Database Links:

BMRB 15408
PDB