BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5154

Title: Backbone dynamics of free N-TIMP-1 in solution   PubMed: 10926526

Authors: Gao, Guanghua; Semenchenko, Valentyna; Arumugam, Sengodagounder; Van Doren, Steven

Citation: Gao, Guanghua; Semenchenko, Valentyna; Arumugam, Sengodagounder; Van Doren, Steven. "Tissue Inhibitor of Metalloproteinases-1 undergoes Microsecond to Millisecond Motions at sites of Matrix Metalloproteinase-induced fit"  J. Mol. Biol. 301, 537-552 (2000).

Assembly members:
N-terminal domain of Tissue Inhibitor of Metalloproteinases-1, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
N-terminal domain of Tissue Inhibitor of Metalloproteinases-1: CTCVPPHPQTAFCNSDLVIR AKFVGTPEVNQTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVCGYFHRSHNRS EEFLIAGKLQDGLLHITTCS FVAPWNSLSLAQRRGFTKTY TVGCEE

Data sets:
Data typeCount
T1 relaxation values102
T2 relaxation values102
order parameters102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-TIMP-1, inhibitor1

Entities:

Entity 1, N-TIMP-1, inhibitor 126 residues - Formula weight is not available

1   CYSTHRCYSVALPROPROHISPROGLNTHR
2   ALAPHECYSASNSERASPLEUVALILEARG
3   ALALYSPHEVALGLYTHRPROGLUVALASN
4   GLNTHRTHRLEUTYRGLNARGTYRGLUILE
5   LYSMETTHRLYSMETTYRLYSGLYPHEGLN
6   ALALEUGLYASPALAALAASPILEARGPHE
7   VALTYRTHRPROALAMETGLUSERVALCYS
8   GLYTYRPHEHISARGSERHISASNARGSER
9   GLUGLUPHELEUILEALAGLYLYSLEUGLN
10   ASPGLYLEULEUHISILETHRTHRCYSSER
11   PHEVALALAPROTRPASNSERLEUSERLEU
12   ALAGLNARGARGGLYPHETHRLYSTHRTYR
13   THRVALGLYCYSGLUGLU

Samples:

sample_1: N-terminal domain of Tissue Inhibitor of Metalloproteinases-1, [U-15N; U-2H], 0.70 mM; sodium acetate, [U-2H], 20 mM; NaCl 150 mM; NaN3 1 mM; H2O 93%; D2O 7%

condition_1: pH: 6.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
15N T1 relaxationnot availablenot availablenot available
15N T2 relaxationnot availablenot availablenot available
heteronuclear 1H-15N NOEnot availablenot availablenot available
transverse cross-relaxation measurementnot availablenot availablenot available

Software:

Sybyl TRIAD v6.3 -

ModelFree v4.1 -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 750 MHz

Related Database Links:

BMRB 4327 5785
PDB
DBJ BAA01913 BAG34878 BAG52016
EMBL CAA26443 CAA26902 CAG28566 CAG46779 CAH90650
GB AAA52436 AAA63234 AAA99943 AAD14009 AAH00866
PIR JC4303
PRF 1107278A 1308125A
REF NP_001028111 NP_001125351 NP_003245 XP_003271098 XP_003917687
SP P01033 P49061 Q5RC60 Q95KL9