BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5155

Title: NMR structure of the UBX domain from P47 (energy minimised average)   PubMed: 11478859

Authors: Yuan, X.; Shaw, A.; Zhang, X.; Kondo, H.; Lally, J.; Freemont, P.; Matthews, S.

Citation: Yuan, X.; Shaw, A.; Zhang, X.; Kondo, H.; Lally, J.; Freemont, P.; Matthews, S.. "Solution Structure and Interaction Surface of the C-terminal Domain from p47: A Major p97-cofactor Involved in SNARE Disassembly "  J. Mol. Biol. 311, 255-263 (2001).

Assembly members:
p47, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
p47: KASSSILINEAEPTTNIQIR LADGGRLVQKFNHSHRISDI RLFIVDARPAMAATSFVLMT TFPNKELADENQTLKEANLL NAVIVQRLT

Data sets:
Data typeCount
1H chemical shifts607
13C chemical shifts174
15N chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p47 protein1

Entities:

Entity 1, p47 protein 89 residues - Formula weight is not available

1   LYSALASERSERSERILELEUILEASNGLU
2   ALAGLUPROTHRTHRASNILEGLNILEARG
3   LEUALAASPGLYGLYARGLEUVALGLNLYS
4   PHEASNHISSERHISARGILESERASPILE
5   ARGLEUPHEILEVALASPALAARGPROALA
6   METALAALATHRSERPHEVALLEUMETTHR
7   THRPHEPROASNLYSGLULEUALAASPGLU
8   ASNGLNTHRLEULYSGLUALAASNLEULEU
9   ASNALAVALILEVALGLNARGLEUTHR

Samples:

sample_1: p47, [U-13C; U-15N], 1 mM; NaAc 20 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.2; temperature: 302 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

AURELIA v2.1.5 - data analysis

NMRPipe - processing

XWINNMR - collection

NMRView v3.1.1 - data analysis

X-PLOR v3.853 - structure solution, refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 5876
PDB
DBJ BAA21659 BAB28604 BAE23234
EMBL CAA71742
GB AAH50936 AAH72464 AAI06102 EDL05910 EDL05911
REF NP_001278003 NP_114187 NP_938085 XP_003500001 XP_003500002
SP O35987 Q9CZ44