BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5162

Title: 1H, 13C, and 15N resonance assignments and secondary structure of the PWI domain from SRm160 using Reduced Dimensionality NMR   PubMed: 11991360

Authors: Szymczyna, Blair; Pineda-Lucena, Antonio; Mills, Jeffrey; Szyperski, Thomas; Arrowsmith, Cheryl

Citation: Szymczyna, Blair; Pineda-Lucena, Antonio; Mills, Jeffrey; Szyperski, Thomas; Arrowsmith, Cheryl. "Letter to the Editor: 1H, 13C, and 15N Resonance Assignments and Secondary Structure of the PWI Domain from SRm160 using Reduced Dimensionality NMR"  J. Biomol. NMR 22, 299-300 (2002).

Assembly members:
SRm160, polymer, 109 residues, 12910 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
SRm160: MQLKFAECLEKKVDMSKVNL EVIKPWITKRVTEILGFEDD VVIEFIFNQLEVKNPDSKMM QINLTGFLNGKNAREFMGEL WPLLLSAQENIAGIPSAFLE LKKEEIKQR

Data sets:
Data typeCount
1H chemical shifts843
13C chemical shifts509
15N chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PWI domain of SRm1601

Entities:

Entity 1, PWI domain of SRm160 109 residues - 12910 Da.

1   METGLNLEULYSPHEALAGLUCYSLEUGLU
2   LYSLYSVALASPMETSERLYSVALASNLEU
3   GLUVALILELYSPROTRPILETHRLYSARG
4   VALTHRGLUILELEUGLYPHEGLUASPASP
5   VALVALILEGLUPHEILEPHEASNGLNLEU
6   GLUVALLYSASNPROASPSERLYSMETMET
7   GLNILEASNLEUTHRGLYPHELEUASNGLY
8   LYSASNALAARGGLUPHEMETGLYGLULEU
9   TRPPROLEULEULEUSERALAGLNGLUASN
10   ILEALAGLYILEPROSERALAPHELEUGLU
11   LEULYSLYSGLUGLUILELYSGLNARG

Samples:

sample_1: SRm160, [U-15N; U-13C], 1.5 mM; sodium phosphate dibasic 7.9 mM; sodium phosphate monobasic 17.1 mM; sodium chloride 300 mM

cond_1: pH: 6.5; temperature: 298 K; ionic strength: 0.34 M

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

PDB
DBJ BAB25575 BAH13726 BAK63562
EMBL CAG31044 CAH92914 CAJ83937
GB AAC09321 AAC17422 AAH36187 AAH74646 AAH82855
REF NP_001005645 NP_001026665 NP_001101456 NP_001123949 NP_001126712
SP Q52KI8 Q5R5Q2 Q5ZMJ9 Q8IYB3
TPG DAA32178