BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5164

Title: NMR Structure of a Parallel Stranded DNA Duplex at Atomic Resolution   PubMed: 11917010

Authors: Parvathy, V.; Bhaumik, S.; Chary, K.; Govil, G.; Liu, K.; Howard, F.; Miles, H.

Citation: Parvathy, V.; Bhaumik, S.; Chary, K.; Govil, G.; Liu, K.; Howard, F.; Miles, H.. "NMR Structure of a Parallel-stranded DNA Duplex at Atomic Resolution"  Nucleic Acids Res. 30, 1500-1511 (2002).

Assembly members:
5'-D(P*CP*CP*AP*TP*AP*AP*TP*TP*TP*AP*CP*C)-3', polymer, 12 residues, Formula weight is not available
5'-D(P*CP*CP*TP*AP*TP*TP*AP*AP*AP*TP*CP*C)-3', polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: .

Entity Sequences (FASTA):
5'-D(P*CP*CP*AP*TP*AP*AP*TP*TP*TP*AP*CP*C)-3': CCATAATTTACC
5'-D(P*CP*CP*TP*AP*TP*TP*AP*AP*AP*TP*CP*C)-3': CCTAAATTATCC

Data sets:
Data typeCount
1H chemical shifts232

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
15'-D(P*CP*CP*AP*TP*AP*AP*TP*TP*TP*AP*CP*C)-3'1
25'-D(P*CP*CP*TP*AP*TP*TP*AP*AP*AP*TP*CP*C)-3'2

Entities:

Entity 1, 5'-D(P*CP*CP*AP*TP*AP*AP*TP*TP*TP*AP*CP*C)-3' 12 residues - Formula weight is not available

1   DCDCDADTDADADTDTDTDA
2   DCDC

Entity 2, 5'-D(P*CP*CP*TP*AP*TP*TP*AP*AP*AP*TP*CP*C)-3' 12 residues - Formula weight is not available

1   DCDCDTDADADADTDTDADT
2   DCDC

Samples:

sample_1: 5'-D(P*CP*CP*AP*TP*AP*AP*TP*TP*TP*AP*CP*C)-3' 5 mM; 5'-D(P*CP*CP*TP*AP*TP*TP*AP*AP*AP*TP*CP*C)-3' 5 mM; sodium acetate buffer, [U-2H], 0.05 M; NaCl 0.1 M; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0.15 M; pH: 5.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
COSYsample_1not availablesample_cond_1
NOESYsample_1not availablesample_cond_1
TOCSYsample_1not availablesample_cond_1

Software:

DISCOVER v3.1 - refinement, structure solution

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links: