BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5165

Title: Solution Structure of Methanobacterium Thermoautotrophicum Protein 1598   PubMed: 11854485

Authors: Yee, A.; Chang, X.; Pineda-Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, K.; Kennedy, M.; Edwards, A.; Arrowsmith, C.

Citation: Yee, A.; Chang, X.; Pineda-Lucena, A.; Wu, B.; Semesi, A.; Le, B.; Ramelot, T.; Lee, G.; Bhattacharyya, S.; Gutierrez, P.; Denisov, A.; Lee, C.; Cort, J.; Kozlov, G.; Liao, J.; Finak, G.; Chen, L.; Wishart, D.; Lee, W.; McIntosh, L.; Gehring, K.; Kennedy, M.; Edwards, A.; Arrowsmith, C.. "An NMR Approach to Structural Proteomics"  Proc. Natl. Acad. Sci. U.S.A. 99, 1825-1830 (2002).

Assembly members:
single chain biopolymer, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Methanothermobacter thermoautotrophicus   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: Euryarchaeota   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
single chain biopolymer: MKGFEFFDVTADAGFWAYGH DLEEVFENAALAMFEVMTDT SLVEAAEERRVEITSEDRVS LLYDWLDELLFIHDTEFILF SKFKVKIDEKDDGLHLTGTA MGEEIKEGHERRDEVKAVTF HMMEILDEDGLIKARVILDL

Data sets:
Data typeCount
1H chemical shifts903
13C chemical shifts585
15N chemical shifts141

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Conserved Hypothetical Protein MTH15981

Entities:

Entity 1, Conserved Hypothetical Protein MTH1598 140 residues - Formula weight is not available

1   METLYSGLYPHEGLUPHEPHEASPVALTHR
2   ALAASPALAGLYPHETRPALATYRGLYHIS
3   ASPLEUGLUGLUVALPHEGLUASNALAALA
4   LEUALAMETPHEGLUVALMETTHRASPTHR
5   SERLEUVALGLUALAALAGLUGLUARGARG
6   VALGLUILETHRSERGLUASPARGVALSER
7   LEULEUTYRASPTRPLEUASPGLULEULEU
8   PHEILEHISASPTHRGLUPHEILELEUPHE
9   SERLYSPHELYSVALLYSILEASPGLULYS
10   ASPASPGLYLEUHISLEUTHRGLYTHRALA
11   METGLYGLUGLUILELYSGLUGLYHISGLU
12   ARGARGASPGLUVALLYSALAVALTHRPHE
13   HISMETMETGLUILELEUASPGLUASPGLY
14   LEUILELYSALAARGVALILELEUASPLEU

Samples:

sample_1: single chain biopolymer, [U-15N; U-13C], 2 mM; NaCl 300 mM; phosphate 10 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.5 na; temperature: 298 K; ionic strength: 300 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
4D 13C-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

NMRPipe v2000.02.14 - processing

Sparky v3.95 - data analysis

CNS v1.0 - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAM70705
GB AAB86071
REF NP_276710 WP_010877206
SP O27635