BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5171

Title: NMR Structure of BPTI Mutant G37A   PubMed: 11841215

Authors: Battiste, J.; Li, R.; Woodward, C.

Citation: Battiste, J.; Li, R.; Woodward, C.. "A Highly Destabilizing Mutation, G37A, of the Bovine Pancreatic Trypsin Inhibitor Retains the Average Native Conformation but Greatly Increases Local Flexibility"  Biochemistry 41, 2237-2245 (2002).

Assembly members:
BPTI, polymer, 58 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: .

Entity Sequences (FASTA):
BPTI: RPDFCLEPPYTGPCKARIIR YFYNAKAGLCQTFVYGACRA KRNNFKSAEDCMRTCGGA

Data sets:
Data typeCount
1H chemical shifts321

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BPTI G37A1

Entities:

Entity 1, BPTI G37A 58 residues - Formula weight is not available

1   ARGPROASPPHECYSLEUGLUPROPROTYR
2   THRGLYPROCYSLYSALAARGILEILEARG
3   TYRPHETYRASNALALYSALAGLYLEUCYS
4   GLNTHRPHEVALTYRGLYALACYSARGALA
5   LYSARGASNASNPHELYSSERALAGLUASP
6   CYSMETARGTHRCYSGLYGLYALA

Samples:

sample_1: BPTI 5 mM; H2O 90%; D2O 10%

sample_2: BPTI 5 mM; D2O 99.9%

sample_cond_1: ionic strength: 30 mM; pH: 4.6; pressure: 1 atm; temperature: 298 K

sample_cond_2: ionic strength: 30 mM; pH: 4.6; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

VNMR v6.1B - data collection

NMRPipe v1.7 - data processing

XEASY v1.2 - data analysis

X-PLOR v3.851 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 1039 1156 1179 18266 236 237 262 263 264 338 411 412 413 414 415 416 419 45 46 48 485 49 4968 5307 5358 5359
PDB
EMBL CAA27062 CAA27063 CAA28371 CAA28886 CAA37967
GB AAB25189 AAD13685 ABX57797 ABX57799
PRF 1405218A 1405218D 681071A
SP P00974