BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5175

Title: Backbone 1H, 13C, 15N and sidechain 1H Chemical shift of CPI-17   PubMed: 11734001

Authors: Ohki, Shin-ya; Eto, Masumi; Kariya, Eri; Hayano, Toshiya; Hayashi, Yuichiro; Yazawa, Michio; Brautigan, David; Kainosho, Masatsune

Citation: Ohki, Shin-ya; Eto, Masumi; Kariya, Eri; Hayano, Toshiya; Hayashi, Yuichiro; Yazawa, Michio; Brautigan, David; Kainosho, Masatsune. "Solution NMR Structure of the Myosin Phosphatase Inhibitor Protein CPI-17 Shows Phosphorylation-induced Conformational Changes Responsible for Activation "  J. Mol. Biol. 314, 839-849 (2001).

Assembly members:
CPI-17, polymer, 86 residues, Formula weight is not available

Natural source:   Common Name: 9823   Taxonomy ID: Pig   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
CPI-17: ARVTVKYDRRELQRRLDVEK WIDGRLEELYRGREADMPDE VNIDELLELESEEERSRKIQ GLLKSCTNPTENFVQELLVK LRGLHK

Data sets:
Data typeCount
1H chemical shifts603
13C chemical shifts168
15N chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CPI-171

Entities:

Entity 1, CPI-17 86 residues - Formula weight is not available

1   ALAARGVALTHRVALLYSTYRASPARGARG
2   GLULEUGLNARGARGLEUASPVALGLULYS
3   TRPILEASPGLYARGLEUGLUGLULEUTYR
4   ARGGLYARGGLUALAASPMETPROASPGLU
5   VALASNILEASPGLULEULEUGLULEUGLU
6   SERGLUGLUGLUARGSERARGLYSILEGLN
7   GLYLEULEULYSSERCYSTHRASNPROTHR
8   GLUASNPHEVALGLNGLULEULEUVALLYS
9   LEUARGGLYLEUHISLYS

Samples:

sample_1: CPI-170.8 – 1.3 mM

Ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 5662 5663
PDB
DBJ BAA22995
REF NP_001179999 NP_999502 XP_004015712 XP_004284289 XP_004330765
SP O18734
TPG DAA19818