BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5177

Title: Backbone and side-chain 1H, 13C, and 15N chemical shift assignments for chick cofilin   PubMed: 11885570

Authors: Bains, Naresh; Gorbatyuk, Vitaliy; Nosworthy, Neil; Robson, Scott; Maciejewski, Mark; dos Remedios, Cristobal; King, Glenn

Citation: Bains, Naresh; Gorbatyuk, Vitaliy; Nosworthy, Neil; Robson, Scott; Maciejewski, Mark; dos Remedios, Cristobal; King, Glenn. "Letter to the Editor: Backbone and Side-chain 1H, 15N, and 13C Assignments for Chick Cofilin"  J. Biomol. NMR 22, 193-194 (2002).

Assembly members:
chick cofilin, polymer, 166 residues, 18662 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
chick cofilin: MASGVTVNDEVIKVFNDMKV RKSSTPEEIKKRKKAVLFCL SDDKKQIIVEEAKQILVGDI GDTVEDPYTAFVKLLPLNDC RYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASS KDAIKKKFTGIKHEWQVNGL DDIKDRSTLGEKLGGNVVVS LEGKPL

Data sets:
Data typeCount
1H chemical shifts1233
13C chemical shifts713
15N chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chick cofilin1

Entities:

Entity 1, chick cofilin 166 residues - 18662 Da.

1   METALASERGLYVALTHRVALASNASPGLU
2   VALILELYSVALPHEASNASPMETLYSVAL
3   ARGLYSSERSERTHRPROGLUGLUILELYS
4   LYSARGLYSLYSALAVALLEUPHECYSLEU
5   SERASPASPLYSLYSGLNILEILEVALGLU
6   GLUALALYSGLNILELEUVALGLYASPILE
7   GLYASPTHRVALGLUASPPROTYRTHRALA
8   PHEVALLYSLEULEUPROLEUASNASPCYS
9   ARGTYRALALEUTYRASPALATHRTYRGLU
10   THRLYSGLUSERLYSLYSGLUASPLEUVAL
11   PHEILEPHETRPALAPROGLUSERALAPRO
12   LEULYSSERLYSMETILETYRALASERSER
13   LYSASPALAILELYSLYSLYSPHETHRGLY
14   ILELYSHISGLUTRPGLNVALASNGLYLEU
15   ASPASPILELYSASPARGSERTHRLEUGLY
16   GLULYSLEUGLYGLYASNVALVALVALSER
17   LEUGLUGLYLYSPROLEU

Samples:

Sample_1: chick cofilin, [U-99% 15N], 1.0 mM; NaCl 50 mM; PIPES 10 mM; H2O 92.5%; D2O 7.5%

Sample_2: chick cofilin, [U-99% 13C; U-99% 15N], 1.0 mM; NaCl 50 mM; PIPES 10 mM; H2O 92.5%; D2O 7.5%

Sample_3: chick cofilin, [U-99% 13C; U-99% 15N], 1.0 mM; NaCl 50 mM; PIPES 10 mM; D2O 100%

Ex-cond_1: pH: 6.8; temperature: 298 K; ionic strength: 0.06 M

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availableEx-cond_1
3D CBCA(CO)NHnot availablenot availableEx-cond_1
3D HNCOnot availablenot availableEx-cond_1
3D HCCH-COSYnot availablenot availableEx-cond_1
3D HCCH-TOCSYnot availablenot availableEx-cond_1
3D HC(CO)NH-TOCSYnot availablenot availableEx-cond_1
3D C(CO)NH-TOCSYnot availablenot availableEx-cond_1
3D 15N-edited NOESY-HSQCnot availablenot availableEx-cond_1
3D 13C-edited NOESY-HSQCnot availablenot availableEx-cond_1
2D (HB)CB(CGCD)HDnot availablenot availableEx-cond_1
2D 1H-1H NOESYnot availablenot availableEx-cond_1
2D 1H-1H DQF-COSYnot availablenot availableEx-cond_1
2D TOCSYnot availablenot availableEx-cond_1

Software:

nmrPipe - NMR data processing

XEASY - Spectral analysis, resonance assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAE27737 BAE87142 BAG36842
GB AAA37433 AAA62732 AAD31280 AAD31281 AAF64498
REF NP_001004406 NP_001020386 NP_001069622 NP_001102452 NP_001162212
SP P21566 P45591 Q148F1 Q5G6V9 Q9Y281
TPG DAA17465