BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5178

Title: Solution Structure of the Fibronectin type III Domain from Bacillus circulans WL-12 Chitinase A1   PubMed: 11600504

Authors: Jee, JunGoo; Ikegami, Takahisa; Hashimoto, Masayuki; Kawabata, Takeshi; Ikeguchi, Mitsunori; Watanabe, Takeshi; Shirakawa, Masahiro

Citation: Jee, JunGoo; Ikegami, Takahisa; Hashimoto, Masayuki; Kawabata, Takeshi; Ikeguchi, Mitsunori; Watanabe, Takeshi; Shirakawa, Masahiro. "Solution Structure of the Fibronectin type III Domain from Bacillus circulans WL-12 Chitinase A1"  J. Biol. Chem. 277, 1388-1397 (2002).

Assembly members:
chitinase FnIII domain, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: soil bacteria   Taxonomy ID: 1397   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus circulans

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
chitinase FnIII domain: HMAPTAPTNLASTAQTTSSI TLSWTASTDNVGVTGYDVYN GTALATTVTGTTATISGLAA DTSYTFTVKAKDAAGNVSAA SNAVSVKT

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts85
1H chemical shifts505

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FnIIID domain 21

Entities:

Entity 1, FnIIID domain 2 88 residues - Formula weight is not available

1   HISMETALAPROTHRALAPROTHRASNLEU
2   ALASERTHRALAGLNTHRTHRSERSERILE
3   THRLEUSERTRPTHRALASERTHRASPASN
4   VALGLYVALTHRGLYTYRASPVALTYRASN
5   GLYTHRALALEUALATHRTHRVALTHRGLY
6   THRTHRALATHRILESERGLYLEUALAALA
7   ASPTHRSERTYRTHRPHETHRVALLYSALA
8   LYSASPALAALAGLYASNVALSERALAALA
9   SERASNALAVALSERVALLYSTHR

Samples:

sample_1: chitinase FnIII domain, [U-15N; U-13C], 1.0 mM; phosphate buffer 20 mM; KCl 50 mM

ex-cond_1: pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz

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