BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5184

Title: 1H Chemical Shift Assignments for toxin BeKm from the Scorpion Buthus eupeus

Authors: Bocharov, Eduard; Maslennikov, Innokenty; Korolkova, Yuliya; Grishin, Eugene; Arseniev, Alexandr

Citation: Bocharov, Eduard; Maslennikov, Innokenty; Korolkova, Yuliya; Grishin, Eugene; Arseniev, Alexandr. "Inverse active site of scorpion toxin BeKm"  Nat. Struct. Biol. ., .-..

Assembly members:
scorpion toxin BeKm, polymer, 36 residues, 4092 Da.

Natural source:   Common Name: lesser Asian scorpion   Taxonomy ID: 34648   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Buthus eupeus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
scorpion toxin BeKm: RPTDIKCSESYQCFPVCKSR FGKTNGRCVNGFCDCF

Data sets:
Data typeCount
1H chemical shifts230
coupling constants30

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BeKm1

Entities:

Entity 1, BeKm 36 residues - 4092 Da.

1   ARGPROTHRASPILELYSCYSSERGLUSER
2   TYRGLNCYSPHEPROVALCYSLYSSERARG
3   PHEGLYLYSTHRASNGLYARGCYSVALASN
4   GLYPHECYSASPCYSPHE

Samples:

sample_1: scorpion toxin BeKm 1.0 mM

sample_cond_1: pH: 3.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H COSYsample_1not availablesample_cond_1
1H TOCSYsample_1not availablesample_cond_1
1H NOESYsample_1not availablesample_cond_1

Software:

XEASY v1.2.11 - peak assignments

DYANA v1.5 - structure calculation

FANTOM v4 - energy minimization

MOLMOL v2.5.1 - visualization, structure analysis

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links:

PDB
GB AAK28021 AIL48783
SP Q9BKB7