BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5187

Title: Assignments of the 1H, 13C, and 15N resonances of TraR   PubMed: 12153045

Authors: Tanaka, Takeshi; Sugai, Mariko; Kobayashi, Kuniko; Kataoka, Masakazu; Kohno, Toshiyuki

Citation: Tanaka, Takeshi; Sugai, Mariko; Kobayashi, Kuniko; Kataoka, Masakazu; Kohno, Toshiyuki. "Letter to the Editor: Complete 1H, 13C, and 15N assignments of the N-terminal DNA binding domain of the TraR protein"  J. Biomol. NMR 23, 161-162 (2002).

Assembly members:
Streptmycal repressor, polymer, 102 residues, 10710 Da.

Natural source:   Common Name: Streptomyces nigrifaciens   Taxonomy ID: 44289   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces nigrifaciens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Streptmycal repressor: GPMPYKAPEGKGYADVATHF RTLIKSGELAPGDTLPSVAD IRAQFGVAAKTVSRALAVLK SEGLVSSRGALGTVVEKNPI VITGADRLKRMEKNGMRYAP GE

Data sets:
Data typeCount
13C chemical shifts389
15N chemical shifts92
1H chemical shifts581

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1TraR1

Entities:

Entity 1, TraR 102 residues - 10710 Da.

1   GLYPROMETPROTYRLYSALAPROGLUGLY
2   LYSGLYTYRALAASPVALALATHRHISPHE
3   ARGTHRLEUILELYSSERGLYGLULEUALA
4   PROGLYASPTHRLEUPROSERVALALAASP
5   ILEARGALAGLNPHEGLYVALALAALALYS
6   THRVALSERARGALALEUALAVALLEULYS
7   SERGLUGLYLEUVALSERSERARGGLYALA
8   LEUGLYTHRVALVALGLULYSASNPROILE
9   VALILETHRGLYALAASPARGLEULYSARG
10   METGLULYSASNGLYMETARGTYRALAPRO
11   GLYGLU

Samples:

sample_1: Streptmycal repressor, [U-99% 13C; U-99% 15N], 1.0 mM

Ex-cond_1: pH: 6.5 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCOnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HBHANHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

AZARA v2.5 -

ANSIG v3.3 -

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAK57424
REF WP_010889977 YP_004797669