BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5193

Title: The solution structure of the mutant 5'AUG3' triloop in the RNA promoter region of the brome mosaic virus genomic (+)-RNA   PubMed: 11680852

Authors: Kim, C.-H.; Kao, C.

Citation: Kim, C.-H.; Kao, C.. "A mutant viral RNA promoter with an altered conformation retains efficient recognition by a viral RNA replicase through a solution-exposed adenine."  RNA 7, 1476-1485 (2001).

Assembly members:
5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3', polymer, 13 residues, Formula weight is not available

Natural source:   Common Name: Brome mosaic virus   Taxonomy ID: 12302   Superkingdom: Viruses   Kingdom: not available   Genus/species: Bromovirus Brome mosaic virus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3': GGUGCAUGGCACC

Data sets:
Data typeCount
1H chemical shifts18

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
15'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3'1

Entities:

Entity 1, 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3' 13 residues - Formula weight is not available

1   GGUGCAUGGC
2   ACC

Samples:

sample_1: 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3' 2 mM; sodium phosphate 10 mM; NaCl 10 mM

sample_2: 5'-R(*GP*GP*UP*GP*CP*AP*UP*GP*GP*CP*AP*CP*C)-3' 2 mM; sodium phosphate 10 mM; NaCl 10 mM; D2O 99.96%

sample_cond_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

sample_cond_2: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

X-PLOR v3.1 - structure solution

xwinnmr - collection

UXNMR - collection

FELIX - data analysis

NMR spectrometers:

  • Bruker AMX 600 MHz

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