BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5199

Title: Assignment of the 1H and 15N resonances of the elicitor protein NIP1

Authors: van, Klaas; 2, 5199; 3, 5199; 4, 5199; 5, 5199; 6, 5199; , 5199

Citation: van, Klaas; 2, 1; 3, 1; 4, 1; 5, 1; 6, 1; , 1. "Solution structure of the fungal plant disease resistance-triggering protein NIP1 shows a novel b sheet fold"  . ., .-..

Assembly members:
Necrosis Inducing Protein 1, polymer, 60 residues, 6434 Da.

Natural source:   Common Name: barley leaf scald fungus   Taxonomy ID: 38038   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Rhynchosporium secalis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Necrosis Inducing Protein 1: DRCRYTLCCDGALKAVSACL HESESCLVPGDCCRGKSRLT LCSYGEGGNGFQCPTGYRQC

Data sets:
Data typeCount
15N chemical shifts65
1H chemical shifts369
coupling constants35

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NIP11

Entities:

Entity 1, NIP1 60 residues - 6434 Da.

1   ASPARGCYSARGTYRTHRLEUCYSCYSASP
2   GLYALALEULYSALAVALSERALACYSLEU
3   HISGLUSERGLUSERCYSLEUVALPROGLY
4   ASPCYSCYSARGGLYLYSSERARGLEUTHR
5   LEUCYSSERTYRGLYGLUGLYGLYASNGLY
6   PHEGLNCYSPROTHRGLYTYRARGGLNCYS

Samples:

sample-1: Necrosis Inducing Protein 1, [U-15N], 2.0 mM

conditions1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYnot availablenot availablenot available
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H CLEAN-TOCSYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D HMQCJnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-15N-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available

Software:

NMRPipe -

XEASY -

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA86496 AAS84297 AAS84298 AAS84299 AAS84300