BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5204

Title: 1H, 13C and 15N chemical shift assignments for CRT(189-261)   PubMed: 12270713

Authors: Ellgaard, Lars; Bettendorff, Pascal; Braun, Daniel; Herrmann, Torsten; Fiorito, Francesco; Guentert, Peter; Helenius, Ari; Wuethrich, Kurt

Citation: Ellgaard, Lars; Bettendorff, Pascal; Braun, Daniel; Herrmann, Torsten; Fiorito, Francesco; Jelesarov, Ilian; Guentert, Peter; Helenius, Ari; Wuethrich, Kurt. "NMR Structures of 36 and 73-residue Fragments of the Calreticulin P-domain"  J. Mol. Biol. 322, 773-784 (2002).

Assembly members:
Calreticulin P-domain fragment, polymer, 74 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Calreticulin P-domain fragment: SKKIKDPDAAKPEDWDERAK IDDPTDSKPEDWDKPEHIPD PDAKKPEDWDEEMDGEWEPP VIQNPEYKGEWKPR

Data sets:
Data typeCount
1H chemical shifts506
13C chemical shifts258
15N chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRT(189-261)1

Entities:

Entity 1, CRT(189-261) 74 residues - Formula weight is not available

1   SERLYSLYSILELYSASPPROASPALAALA
2   LYSPROGLUASPTRPASPGLUARGALALYS
3   ILEASPASPPROTHRASPSERLYSPROGLU
4   ASPTRPASPLYSPROGLUHISILEPROASP
5   PROASPALALYSLYSPROGLUASPTRPASP
6   GLUGLUMETASPGLYGLUTRPGLUPROPRO
7   VALILEGLNASNPROGLUTYRLYSGLYGLU
8   TRPLYSPROARG

Samples:

sample_1: Calreticulin P-domain fragment, [U-15N; U-13C], 3 mM; sodium phosphate buffer 50 mM; CaCl2 10 mM; D20 25 ul; H20 275 ul

condition_1: pH: 6.3; temperature: 293.4 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D 13C-1H-1H NOESYnot availablenot availablenot available
3D COMBINED 1H-1H-13C/15N NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 17224 4878
PDB
DBJ BAA11345 BAB86913 BAC35852 BAD96780 BAE01267
EMBL CAA33053 CAA37446 CAA55890 CAG33351
GB AAA31188 AAA36582 AAA37569 AAA51916 AAB20096
REF NP_001075704 NP_001167604 NP_001231051 NP_001248060 NP_001274539
SP P14211 P15253 P18418 P27797 P28491
TPG DAA28020