BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5205

Title: 1H chemical shift assignemnts and coupling constants for CRT(221-256)   PubMed: 12270713

Authors: Ellgaard, Lars; Bettendorff, Pascal; Braun, Daniel; Herrmann, Torsten; Fiorito, Francesco; Guentert, Peter; Helenius, Ari; Wuethrich, Kurt

Citation: Ellgaard, Lars; Bettendorff, Pascal; Braun, Daniel; Herrmann, Torsten; Fiorito, Francesco; Jelesarov, Ilian; Guentert, Peter; Helenius, Ari; Wuethrich, Kurt. "NMR Structures of 36 and 73-residue Fragments of the Calreticulin P-domain"  J. Mol. Biol. 322, 773-784 (2002).

Assembly members:
Calreticulin P-domain fragment., polymer, 36 residues, Formula weight is not available

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Calreticulin P-domain fragment.: KPEHIPDPDAKKPEDWDEEM DGEWEPPVIQNPEYKG

Data sets:
Data typeCount
1H chemical shifts246
coupling constants54

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CRT(221-256)1

Entities:

Entity 1, CRT(221-256) 36 residues - Formula weight is not available

1   LYSPROGLUHISILEPROASPPROASPALA
2   LYSLYSPROGLUASPTRPASPGLUGLUMET
3   ASPGLYGLUTRPGLUPROPROVALILEGLN
4   ASNPROGLUTYRLYSGLY

Samples:

sample_1: Calreticulin P-domain fragment. 5.3 mM; sodium phosphate buffer 50 mM; NaCl 25 mM; D20 50 ul; H20 550 ul

condition_1: pH: 6.5; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESY, mixing time 60 mssample_1not availablecondition_1
2D 1H-1H TOCSY, mixing time 100 mssample_1not availablecondition_1
2D 1H-1H COSY-DQFsample_1not availablecondition_1
2D 1H-1H E.COSYsample_1not availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 17224 4878 5204
PDB
DBJ BAA11345 BAB86913 BAC35852 BAD96780 BAE01267
EMBL CAA33053 CAA37446 CAA47866 CAA55890 CAG33351
GB AAA31188 AAA36582 AAA37569 AAA51916 AAB20096
REF NP_001075704 NP_001080096 NP_001167604 NP_001231051 NP_001248060
SP P14211 P15253 P18418 P27797 P28491
TPG DAA28020