BMRB Entry 5207

Title:
1H Chemical Shifts for the Apo State of the F36G + P43M Mutant of Calbindin D9k
Deposition date:
2001-11-14
Original release date:
2002-02-14
Authors:
Nelson, Melanie; Thulin, Eva; Fagan, Patricia; Forsen, Sture; Chazin, Walter
Citation:

Citation: Nelson, Melanie; Thulin, Eva; Fagan, Patricia; Forsen, Sture; Chazin, Walter. "The EF-hand Domain: A Globally Cooperative Structural unit"  Protein Sci. 11, 198-205 (2002).
PubMed: 11790829

Assembly members:

Assembly members:
CALBINDIN D9K, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRCB1

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts518

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CALBINDIN D9K F36G1

Entities:

Entity 1, CALBINDIN D9K F36G 76 residues - Formula weight is not available

1   METLYSSERPROGLUGLULEULYSGLYILE
2   PHEGLULYSTYRALAALALYSGLUGLYASP
3   PROASNGLNLEUSERLYSGLUGLULEULYS
4   LEULEULEUGLNTHRGLUGLYPROSERLEU
5   LEULYSGLYMETSERTHRLEUASPGLULEU
6   PHEGLUGLULEUASPLYSASNGLYASPGLY
7   GLUVALSERPHEGLUGLUPHEGLNVALLEU
8   VALLYSLYSILESERGLN

Samples:

sample_1: CALBINDIN D9K 2.5 mM; H2O 100%

sample_2: CALBINDIN D9K 2.5 mM; D2O 100%

sample_3: CALBINDIN D9K, [U-15N], 2.5 mM

sample_cond_1: pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D COSYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
2D 15N-1H HSQCnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 15N-separated TOCSYnot availablenot availablesample_cond_1
3D HNHAnot availablenot availablesample_cond_1
3D HNHBnot availablenot availablesample_cond_1

Software:

FELIX v97.0 - data processing, resonance assignments

DIANA v2.8 - calculation of starting structures

AMBER v4.1 - structure refinement

GENXPK v1 - resonance and NOE assignments

GLOMSA - determine dihedral angle constraints

NMR spectrometers:

  • BRUKER DMX 750 MHz
  • BRUKER AMX 500 MHz

Related Database Links:

BMRB 15594 16340 247 325 326 327 4581 6697 6699 753 939 940
PDB
GB AAA30420 AAA72542 AAI18481
PRF 0707237A:PDB=3ICB
REF NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038
SP P02633
TPG DAA12577
AlphaFold P02633