BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5208

Title: 1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase B   PubMed: 11883786

Authors: Gryk, Michael; Maciejewski, Mark; Robertson, Anthony; Mullen, Mary; Wilson, Samuel; Mullen, Gregory

Citation: Gryk, Michael; Maciejewski, Mark; Robertson, Anthony; Mullen, Mary; Wilson, Samuel; Mullen, Gregory. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments for the Perdeuterated 22 kD Palm-thumb Domain of DNA Polymerase Beta"  J. Biomol. NMR 22, 197-198 (2002).

Assembly members:
DNA Polymerase B, polymer, 190 residues, 22022 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DNA Polymerase B: MGKRIPREEMLQMQDIVLNE VKKLDPEYIATVCGSFRRGA ESSGDMDVLLTHPNFTSESS KQPKLLHRVVEQLQKVRFIT DTLSKGETKFMGVCQLPSEN DENEYPHRRIDIRLIPKDQY YCGVLYFTGSDIFNKNMRAH ALEKGFTINEYTIRPLGVTG VAGEPLPVDSEQDIFDYIQW RYREPKDRSE

Data sets:
Data typeCount
1H chemical shifts354
13C chemical shifts621
15N chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1B-Pol palm-thumb1

Entities:

Entity 1, B-Pol palm-thumb 190 residues - 22022 Da.

1   METGLYLYSARGILEPROARGGLUGLUMET
2   LEUGLNMETGLNASPILEVALLEUASNGLU
3   VALLYSLYSLEUASPPROGLUTYRILEALA
4   THRVALCYSGLYSERPHEARGARGGLYALA
5   GLUSERSERGLYASPMETASPVALLEULEU
6   THRHISPROASNPHETHRSERGLUSERSER
7   LYSGLNPROLYSLEULEUHISARGVALVAL
8   GLUGLNLEUGLNLYSVALARGPHEILETHR
9   ASPTHRLEUSERLYSGLYGLUTHRLYSPHE
10   METGLYVALCYSGLNLEUPROSERGLUASN
11   ASPGLUASNGLUTYRPROHISARGARGILE
12   ASPILEARGLEUILEPROLYSASPGLNTYR
13   TYRCYSGLYVALLEUTYRPHETHRGLYSER
14   ASPILEPHEASNLYSASNMETARGALAHIS
15   ALALEUGLULYSGLYPHETHRILEASNGLU
16   TYRTHRILEARGPROLEUGLYVALTHRGLY
17   VALALAGLYGLUPROLEUPROVALASPSER
18   GLUGLNASPILEPHEASPTYRILEGLNTRP
19   ARGTYRARGGLUPROLYSASPARGSERGLU

Samples:

perdeuterated: DNA Polymerase B, U-98% 13C; U-99% 15N; U-97% 2H; except V g-methyls, L d-methyls, I d-methyl, and labile protons. , 46; 5208, 1, 135; 5208, 1, 0.002; 5208, 1, 100; 5208, 1, 1; 5208, 1, 90; 5208, 1, 10; 5208, 1,

perdeuterated_cond1: pH: 6.8 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D TROSY 1H-15N HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D CT 1H-13C HSQCnot availablenot availablenot available
3D TROSY HNCACBnot availablenot availablenot available
3D TROSY HNCOnot availablenot availablenot available
3D TROSY HN(CA)COnot availablenot availablenot available
2D CCH-TOCSYnot availablenot availablenot available
3D (HM)CMC(CM)HMnot availablenot availablenot available
3D 15N,13C NOESYnot availablenot availablenot available

Software:

NMRPipe v1.8 - spectral processing

sbtools - NMR data processing script generation

XEASY v1.2 - spectral analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18267 7319
PDB
GB AAA41900 AAA41901 AAB00389 AAH98668 EDM09009
REF NP_058837
SP P06766