BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5210

Title: NMR Solution Structure of sTva47, the Viral-Binding Domain of Tva   PubMed: 11768384

Authors: Tonelli, M.; Peters, R.; James, T.; Agard, D.

Citation: Tonelli, M.; Peters, R.; James, T.; Agard, D.. "The Solution Structure of the Viral-Binding Domain of Tva, the Cellular Receptor for Subgroup A Avian Leukosis and Sarcoma Virus"  FEBS Lett. 509, 161-168 (2001).

Assembly members:
subgroup A Rous Sarcoma virus receptors, polymer, 47 residues, Formula weight is not available

Natural source:   Common Name: Quail   Taxonomy ID: 9008   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Callipepla not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
subgroup A Rous Sarcoma virus receptors: ISEFGSCPPGQFRCSEPPGA HGECYPQDWLCDGHPDCDDG RDEWGCG

Data sets:
Data typeCount
13C chemical shifts135
15N chemical shifts44
1H chemical shifts245

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subgroup A Rous Sarcoma virus receptors1

Entities:

Entity 1, subgroup A Rous Sarcoma virus receptors 47 residues - Formula weight is not available

1   ILESERGLUPHEGLYSERCYSPROPROGLY
2   GLNPHEARGCYSSERGLUPROPROGLYALA
3   HISGLYGLUCYSTYRPROGLNASPTRPLEU
4   CYSASPGLYHISPROASPCYSASPASPGLY
5   ARGASPGLUTRPGLYCYSGLY

Samples:

sample_1: subgroup A Rous Sarcoma virus receptors, [U-15N; U-13C], 2 mM; sodium acetate, [U-2H], 50 mM; calcium cloride 5 mM; D2O 10%; H2O 90%

sample_cond_1: ionic strength: 0.065 M; pH: 5.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablesample_cond_1
3D 13C-separated NOESYsample_1not availablesample_cond_1

Software:

VNMR v6.1 - collection

NMRPipe vsgi6x - processing

SPARKY v3 - data analysis

DYANA v1.5 - refinement

AMBER v6.0 - refinement

NMR spectrometers:

  • Varian UnityPlus 600 MHz
  • Bruker DMX 600 MHz

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