BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5211

Title: SAP/SH2D1A bound to peptide n-Y-c   PubMed: 11823424

Authors: Hwang, P.; Li, C.; Morra, M.; Lillywhite, J.; Muhandiram, D.; Gertler, F.; Terhorst, C.; Kay, L.; Pawson, T.; Forman-Kay, J.; Li, S.-C.

Citation: Hwang, P.; Li, C.; Morra, M.; Lillywhite, J.; Muhandiram, D.; Gertler, F.; Terhorst, C.; Kay, L.; Pawson, T.; Forman-Kay, J.; Li, S.-C.. "A "three-pronged" Binding Mechanism for the SAP/SH2D1A SH2 Domain: Structural Basis and Relevance to the XLP Syndrome "  EMBO J. 21, 314-323 (2002).

Assembly members:
SAP/SH2D1A, polymer, 128 residues, Formula weight is not available
peptide n-Y-c, polymer, 12 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
SAP/SH2D1A: MDAVAVYHGKISRETGEKLL LATGLDGSYLLRDSESVPGV YCLCVLYHGYIYTYRVSQTE TGSWSAETAPGVHKRYFRKI KNLISAFQKPDQGIVIPLQY PVEKKSSARSTQGTTGIRED PDVCLKAP
peptide n-Y-c: RKSLTIYAQVQK

Data sets:
Data typeCount
1H chemical shifts953
13C chemical shifts554
15N chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH2 DOMAIN PROTEIN 1A1
2peptide n-Y-c2

Entities:

Entity 1, SH2 DOMAIN PROTEIN 1A 128 residues - Formula weight is not available

1   METASPALAVALALAVALTYRHISGLYLYS
2   ILESERARGGLUTHRGLYGLULYSLEULEU
3   LEUALATHRGLYLEUASPGLYSERTYRLEU
4   LEUARGASPSERGLUSERVALPROGLYVAL
5   TYRCYSLEUCYSVALLEUTYRHISGLYTYR
6   ILETYRTHRTYRARGVALSERGLNTHRGLU
7   THRGLYSERTRPSERALAGLUTHRALAPRO
8   GLYVALHISLYSARGTYRPHEARGLYSILE
9   LYSASNLEUILESERALAPHEGLNLYSPRO
10   ASPGLNGLYILEVALILEPROLEUGLNTYR
11   PROVALGLULYSLYSSERSERALAARGSER
12   THRGLNGLYTHRTHRGLYILEARGGLUASP
13   PROASPVALCYSLEULYSALAPRO

Entity 2, peptide n-Y-c 12 residues - Formula weight is not available

1   ARGLYSSERLEUTHRILETYRALAGLNVAL
2   GLNLYS

Samples:

sample_1: SAP/SH2D1A, [U-15N; U-13C], 1 mM; peptide n-Y-c 1 mM; phosphate buffer 20 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0; temperature: 303 K; ionic strength: 120 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N,13C-separated NOESYnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D HN(CO)HBnot availablenot availablenot available

Software:

ARIA v1.0 - structure solution

NMRPipe v1.8 - processing

NMRView v3.0 - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWISS-PROT O60880
REF XP_865096 NP_002342 NP_001028009
GenBank AAK11578 AAH20732 AAC79712 AAC62631 AAC62630
EMBL CAG46840 CAG46828 CAA19222 CAA18777
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