BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5213

Title: Copper Trafficking: The Solution Structure of Bacillus subtilis CopZ   PubMed: 11747441

Authors: Banci, L.; Bertini, I.; Del Conte, R.; Markey, J.; Ruiz-Duenas, J.

Citation: Banci, L.; Bertini, I.; Del Conte, R.; Markey, J.; Ruiz-Duenas, F.. "Copper Trafficking: The Solution Structure of Bacillus subtilis CopZ"  Biochemistry 40, 15660-15668 (2001).

Assembly members:
CopZ from Bacillus subtilis, polymer, 73 residues, 7826 Da.
COPPER (I) ION, non-polymer, 63.546 Da.

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Bacillus subtilis

Entity Sequences (FASTA):
CopZ from Bacillus subtilis: MEQKTLQVEGMSCQHCVKAV ETSVGELDGVSAVHVNLEAG KVDVSFDADKVSVKDIADAI EDQGYDVAKIEGR

Data sets:
Data typeCount
15N chemical shifts73
1H chemical shifts461

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CopZ from Bacillus subtilis1
2COPPER (I) ION2

Entities:

Entity 1, CopZ from Bacillus subtilis 73 residues - 7826 Da.

1   METGLUGLNLYSTHRLEUGLNVALGLUGLY
2   METSERCYSGLNHISCYSVALLYSALAVAL
3   GLUTHRSERVALGLYGLULEUASPGLYVAL
4   SERALAVALHISVALASNLEUGLUALAGLY
5   LYSVALASPVALSERPHEASPALAASPLYS
6   VALSERVALLYSASPILEALAASPALAILE
7   GLUASPGLNGLYTYRASPVALALALYSILE
8   GLUGLYARG

Entity 2, COPPER (I) ION - Cu - 63.546 Da.

1   CU1

Samples:

sample_1: CopZ from Bacillus subtilis, [U-99% 15N], 2 mM; COPPER (I) ION 2 mM; phosphate buffer 100 mM; DTT trace mM; D2O 10%; H2O 90%

sample_2: CopZ from Bacillus subtilis 2 mM; COPPER (I) ION 2 mM; phosphate buffer 100 mM; DTT trace mM; D2O 10%; H2O 90%

sample_cond_1: pH: 7.0 na; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D TOSCY-HMQCnot availablenot availablesample_cond_1
3D NOESY-HSQCnot availablenot availablesample_cond_1
3D HNHAnot availablenot availablesample_cond_1
2D TOSCYnot availablenot availablesample_cond_1
2D NOESYnot availablenot availablesample_cond_1
2D HSQCnot availablenot availablesample_cond_1

Software:

DYANA v1.5 - solution structure calculation

xwinnmr v2.6 - NMR experiments collection

XEASY v1.3.13 - data analysis

AMBER v5.0 - solution structure refinement

Molmol v2.4 - data analysis

CORMA - data analysis

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links: