BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5220

Title: Solution Structure and dynamics of melanoma inhibitory activity protein

Authors: Lougheed, J.; Domaille, P.; Handel, T.

Citation: Lougheed, J.; Domaille, P.; Handel, T.. "Solution Structure and Dynamics of Melanoma Inhibitory Activity Protein"  J. Biomol. NMR 22, 211-223 (2002).

Assembly members:
melanoma inhibitory activity protein, polymer, 108 residues, 12237 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
melanoma inhibitory activity protein: MGPMPKLADRKLCADQECSH PISMAVALQDYMAPDCRFLT IHRGQVVYVFSKLKGRGRLF WGGSVQGDYYGDLAARLGYF PSSIVREDQTLKPGKVDVKT DKWDFYCQ

Data sets:
Data typeCount
1H chemical shifts764
13C chemical shifts386
15N chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Melanoma Inhibitory Activity Protein1

Entities:

Entity 1, Melanoma Inhibitory Activity Protein 108 residues - 12237 Da.

1   METGLYPROMETPROLYSLEUALAASPARG
2   LYSLEUCYSALAASPGLNGLUCYSSERHIS
3   PROILESERMETALAVALALALEUGLNASP
4   TYRMETALAPROASPCYSARGPHELEUTHR
5   ILEHISARGGLYGLNVALVALTYRVALPHE
6   SERLYSLEULYSGLYARGGLYARGLEUPHE
7   TRPGLYGLYSERVALGLNGLYASPTYRTYR
8   GLYASPLEUALAALAARGLEUGLYTYRPHE
9   PROSERSERILEVALARGGLUASPGLNTHR
10   LEULYSPROGLYLYSVALASPVALLYSTHR
11   ASPLYSTRPASPPHETYRCYSGLN

Samples:

sample_1: melanoma inhibitory activity protein, [U-13C; U-15N], 0.7 – 0.9 mM; sodium acetate, [U-2H], 20 mM; azide 0.02%

sample_cond_1: pH: 4.33; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm

sample_cond_2: pH*: 4.33; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D CBCANNHnot availablenot availablenot available
3D CBCA(CO)NNHnot availablenot availablenot available
4D HCC(CO)NNHnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
2D CBHDnot availablenot availablenot available
2D CBHEnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
4D 13C/15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
4D 13C-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESY(centered on aromatic carbons)not availablenot availablenot available
3D 1H-TOCSY-relayed ct-[13C, 1H]-HMQCnot availablenot availablenot available

Software:

X-PLOR v3.851 - structure solution, refinement

ARIA - structure calculation, automated assignment of NOEs

ANSIG v3.3 - data analysis

AZARA v2.0 - processing

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4731
PDB
DBJ BAG63518
EMBL CAA53203 CAA59195 CAG46449
GB AAF76220 AAH05910 AAP35693 AAP36443 AAX29533
REF NP_001189482 NP_001248211 NP_006524 XP_001146858 XP_002829285
SP Q16674