BMRB Entry 52246

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52246

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Title:
1H and 15N Chemical Shifts of alpha7nAChR ICD without PICK1
Deposition date:
2023-12-20
Original release date:
2024-05-02
Authors:
Bondarenko, Vasyl; Chen, Qiang; Tang, Pei
Citation:

Citation: Bondarenko, Vasyl; Chen, Qiang; Tillman, Tommy; Xu, Yan; Tang, Pei. "Unconventional PDZ Recognition Revealed in a7 nAChR-PICK1 Complexes"  ACS Chem. Neurosci. 15, 2070-2079 (2024).
PubMed: 38691676

Assembly members:

Assembly members:
entity_1, polymer, 272 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTBSG1-TMD+ICD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNAEEELYYGLNLLIPCVLI SALALLVFLLPADSGEKISL GITVLLSLTVFMLLVAEIMP STSDSSPSIAQYFASTMIIV GLSVVVTVIVLQYHHHDPDG GKMPKWTRVILLNWCAWFLR MKRPGEDKVRPACQHKQRRC SLASVEMSAVAPPPASNGNL LYIGFRGLDGVHCVPTPDSG VVCGRMACSPTHDEHLLHGG QPPEGDPDLAKILEEVRYIA NRFRCQDESEAVCSEWKFAA CVVDRLCLMAFSVFTIICTI GILMSAPNFVEE

Data sets:
Data typeCount
15N chemical shifts129
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha7ACHR, chain A1
2alpha7ACHR, chain B1
3alpha7ACHR, chain C1
4alpha7ACHR, chain D1
5alpha7ACHR, chain E1

Entities:

Entity 1, alpha7ACHR, chain A 272 residues - Formula weight is not available

Intracellular and transmembrane domains of human alpha7 nicotinic acetylcholine receptor: chain A, B, C, D, E

1   SERASNALAGLUGLUGLULEUTYRTYRGLY
2   LEUASNLEULEUILEPROCYSVALLEUILE
3   SERALALEUALALEULEUVALPHELEULEU
4   PROALAASPSERGLYGLULYSILESERLEU
5   GLYILETHRVALLEULEUSERLEUTHRVAL
6   PHEMETLEULEUVALALAGLUILEMETPRO
7   SERTHRSERASPSERSERPROSERILEALA
8   GLNTYRPHEALASERTHRMETILEILEVAL
9   GLYLEUSERVALVALVALTHRVALILEVAL
10   LEUGLNTYRHISHISHISASPPROASPGLY
11   GLYLYSMETPROLYSTRPTHRARGVALILE
12   LEULEUASNTRPCYSALATRPPHELEUARG
13   METLYSARGPROGLYGLUASPLYSVALARG
14   PROALACYSGLNHISLYSGLNARGARGCYS
15   SERLEUALASERVALGLUMETSERALAVAL
16   ALAPROPROPROALASERASNGLYASNLEU
17   LEUTYRILEGLYPHEARGGLYLEUASPGLY
18   VALHISCYSVALPROTHRPROASPSERGLY
19   VALVALCYSGLYARGMETALACYSSERPRO
20   THRHISASPGLUHISLEULEUHISGLYGLY
21   GLNPROPROGLUGLYASPPROASPLEUALA
22   LYSILELEUGLUGLUVALARGTYRILEALA
23   ASNARGPHEARGCYSGLNASPGLUSERGLU
24   ALAVALCYSSERGLUTRPLYSPHEALAALA
25   CYSVALVALASPARGLEUCYSLEUMETALA
26   PHESERVALPHETHRILEILECYSTHRILE
27   GLYILELEUMETSERALAPROASNPHEVAL
28   GLUGLU

Samples:

sample_1: Neuronal acetylcholine receptor subunit alpha7 for binding PICK1, [U-100% 15N], 0.2 ± 0.01 mM; sodium acetate 5 mM; sodium chloride 25 mM; DSS 20 uM; LDAO 20 mM

sample_conditions_1: ionic strength: 25 mM; pH: 5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2 - collection

NMRPipe v10.9 - processing

NMRFAM-SPARKY v1.470 - data analysis

SPARKY v3.190 - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks