BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5225

Title: Solution Structure of the Peptidyl-Prolyl-cis/trans-Isomerase Parvulin 10 of Escherichia coli

Authors: Kuhlewein, Angelika; Voll, Georg; Schelbert, Birte; Kessler, Horst; Fischer, Gunter; Rahfeld, Jens; Gemmecker, Gerd

Citation: Kuhlewein, Angelika; Voll, Georg; Schelbert, Birte; Kessler, Horst; Fischer, Gunter; Rahfeld, Jens; Gemmecker, Gerd. "Solution Structure of the Peptidyl-Prolyl-cis/trans-Isomerase Parvulin 10 of Escherichia coli "  J. Mol. Biol. ., .-..

Assembly members:
Parvulin 10, polymer, 92 residues, 10101 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Parvulin 10: AKTAAALHILVKEEKLALDL LEQIKNGADFGKLAKKHSIC PSGKRGGDLGEFRQGQMVPA FDKVVFSCPVLEPTGPLHTQ FGYHIIKVLYRN

Data sets:
Data typeCount
1H chemical shifts621
13C chemical shifts408
15N chemical shifts89
coupling constants69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1parvulin 101

Entities:

Entity 1, parvulin 10 92 residues - 10101 Da.

1   ALALYSTHRALAALAALALEUHISILELEU
2   VALLYSGLUGLULYSLEUALALEUASPLEU
3   LEUGLUGLNILELYSASNGLYALAASPPHE
4   GLYLYSLEUALALYSLYSHISSERILECYS
5   PROSERGLYLYSARGGLYGLYASPLEUGLY
6   GLUPHEARGGLNGLYGLNMETVALPROALA
7   PHEASPLYSVALVALPHESERCYSPROVAL
8   LEUGLUPROTHRGLYPROLEUHISTHRGLN
9   PHEGLYTYRHISILEILELYSVALLEUTYR
10   ARGASN

Samples:

sample_1: Parvulin 10, [U-15N], 0.8 mM; KCl 100 mM; phosphate buffer 10 mM

sample_2: Parvulin 10, [U-13C; U-15N], 0.8 mM; KCl 100 mM; phosphate buffer 10 mM

conditions_1: pH: 6.0; temperature: 297.6 K; ionic strength: 0.13 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HA(CACO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D 15N-edited TOCSYnot availablenot availablenot available
(H)CC(CO)NHnot availablenot availablenot available
MEXICOnot availablenot availablenot available
3D 15N-edited NOESYnot availablenot availablenot available
3D 13C-edited NOESYnot availablenot availablenot available
3D double 13C-edited NOESYnot availablenot availablenot available
3D 13C,15N-edited NOESYnot availablenot availablenot available

Software:

XWINNMR v2.6 - data acquisition, data processing

SYBYL_TRIAD v6.6 - peak picking, NOE assignment

PASTA v1.0/2.0/3.0b - sequential backbone assignment

NMR spectrometers:

  • BRUKER DMX 600 MHz
  • BRUKER DMX 750 MHz

Related Database Links:

PDB
DBJ BAB38132 BAE77522 BAG79582 BAI27969 BAI33092
EMBL CAP78224 CAQ34119 CAQ91187 CAR00747 CAR05395
GB AAA67578 AAB32054 AAC76780 AAG58970 AAN45287
REF NP_290406 NP_312736 NP_418223 NP_709580 NP_756555
SP P0A9L5 P0A9L6 P0A9L7