BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5232

Title: 1H, 13C, and 15N resonance assignments of the DNA-binding domain of the essential protein Cdc13 complexed with single-stranded telomeric DNA   PubMed: 12018489

Authors: Mitton-Fry, Rachel; Wuttke, Deborah

Citation: Mitton-Fry, Rachel; Wuttke, Deborah. "Letter to the Editor: 1H, 13C, and 15N resonance assignments of the DNA-binding domain of the essential protein Cdc13 complexed with single-stranded telomeric DNA "  J. Biomol. NMR 22, 379-380 (2002).

Assembly members:
Cdc13 DNA-binding domain, polymer, 199 residues, Formula weight is not available
telomeric single-stranded DNA 11mer, polymer, 11 residues, Formula weight is not available

Natural source:   Common Name: Baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Cdc13 DNA-binding domain: MRMSKMARKDPTIEFCQLGL DTFETKYITMFGMLVSCSFD KPAFISFVFSDFTKNDIVQN YLYDRYLIDYENKLELNEGF KAIMYKNQFETFDSKLRKIF NNGLRDLQNGRDENLSQYGI VCKMNIKVKMYNGKLNAIVR ECEPVPHSQISSIASPSQCE HLRLFYQRAFKRIGESAISR YFEEYRRFFPIHRNGSHLA
telomeric single-stranded DNA 11mer: GTGTGGGTGTG

Data sets:
Data typeCount
1H chemical shifts1238
13C chemical shifts823
15N chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc13 DNA-binding Domain1
2telomeric single-stranded DNA2

Entities:

Entity 1, Cdc13 DNA-binding Domain 199 residues - Formula weight is not available

1   METARGMETSERLYSMETALAARGLYSASP
2   PROTHRILEGLUPHECYSGLNLEUGLYLEU
3   ASPTHRPHEGLUTHRLYSTYRILETHRMET
4   PHEGLYMETLEUVALSERCYSSERPHEASP
5   LYSPROALAPHEILESERPHEVALPHESER
6   ASPPHETHRLYSASNASPILEVALGLNASN
7   TYRLEUTYRASPARGTYRLEUILEASPTYR
8   GLUASNLYSLEUGLULEUASNGLUGLYPHE
9   LYSALAILEMETTYRLYSASNGLNPHEGLU
10   THRPHEASPSERLYSLEUARGLYSILEPHE
11   ASNASNGLYLEUARGASPLEUGLNASNGLY
12   ARGASPGLUASNLEUSERGLNTYRGLYILE
13   VALCYSLYSMETASNILELYSVALLYSMET
14   TYRASNGLYLYSLEUASNALAILEVALARG
15   GLUCYSGLUPROVALPROHISSERGLNILE
16   SERSERILEALASERPROSERGLNCYSGLU
17   HISLEUARGLEUPHETYRGLNARGALAPHE
18   LYSARGILEGLYGLUSERALAILESERARG
19   TYRPHEGLUGLUTYRARGARGPHEPHEPRO
20   ILEHISARGASNGLYSERHISLEUALA

Entity 2, telomeric single-stranded DNA 11 residues - Formula weight is not available

1   DGDTDGDTDGDGDGDTDGDT
2   DG

Samples:

sample_1: Cdc13 DNA-binding domain, [U-13C; U-15N], 0.7 – 1.1 mM; telomeric single-stranded DNA 11mer0.8 – 1.2 mM; imidazole, [U-2H], 50 mM; sodium chloride 150 mM; sodium sulfate 100 mM; sodium azide 0.02%; DTT, [U-2H], 2 mM

sample_2: Cdc13 DNA-binding domain, [U-15N], 1.5 mM; telomeric single-stranded DNA 11mer 1.7 mM; imidazole, [U-2H], 50 mM; sodium chloride 150 mM; sodium sulfate 100 mM; sodium azide 0.02%; DTT, [U-2H], 2 mM

sample_3: Cdc13 DNA-binding domain 1.1 mM; telomeric single-stranded DNA 11mer 1.2 mM; imidazole, [U-2H], 50 mM; sodium chloride 150 mM; sodium sulfate 100 mM; sodium azide 0.02%; DTT, [U-2H], 2 mM

Ex-cond_1: pH: 7.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-13C CT-HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D CBHDnot availablenot availablenot available
2D CBHEnot availablenot availablenot available
3D 1H-13C-1H HCCH-COSYnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 13C-1H-1H NOESYnot availablenot availablenot available
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HCACOnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available
3D C(CO)NHnot availablenot availablenot available
3D HC(CO)NHnot availablenot availablenot available
4D 1H-15N-1H-13C NOESYnot availablenot availablenot available
4D 1H-13C-13C-1H NOESYnot availablenot availablenot available

Software:

NMRPipe - data processing

Ansig v3.3 - data analysis

NMR spectrometers:

  • Varian UnityInova 500 MHz
  • Varian UnityInova 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ GAA22033
EMBL CAA98800 CAY79065
GB AAA99990 AHY74797 EDN60143 EDV08479 EDZ73439
REF NP_010061
SP P32797
TPG DAA11644