BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5236

Title: 1H and 15N Chemical Shift Assignments for the tandem inactivation domain of Kv1.4 (RCK4(1-75))   PubMed: 12590144

Authors: Wissmann, Ralph; Bildl, Wolfgang; Oliver, Dominik; Jonas, Peter; Bentrop, Detlef; Fakler, Bernd

Citation: Wissmann, Ralph; Bildl, Wolfgang; Oliver, Dominik; Beyermann, M.; Kalbitzer, H.; Bentrop, Detlef; Fakler, Bernd. "Solution Structure and Function of the "Tandem Inactivation Domain" of the Neuronal A-type Potassium Channel Kv1.4."  J. Biol. Chem. 278, 16142-16150 (2003).

Assembly members:
RCK4, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RCK4: GSTMEVAMVSAESSGCNSHM PYGYAAQARARERERLAHSR AAAAAAVAAATAAVEGTGGS GGGPHHHHQTRGAYSSHD

Data sets:
Data typeCount
1H chemical shifts397
15N chemical shifts76
coupling constants62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RCK4(1-75)1

Entities:

Entity 1, RCK4(1-75) 78 residues - Formula weight is not available

1   GLYSERTHRMETGLUVALALAMETVALSER
2   ALAGLUSERSERGLYCYSASNSERHISMET
3   PROTYRGLYTYRALAALAGLNALAARGALA
4   ARGGLUARGGLUARGLEUALAHISSERARG
5   ALAALAALAALAALAALAVALALAALAALA
6   THRALAALAVALGLUGLYTHRGLYGLYSER
7   GLYGLYGLYPROHISHISHISHISGLNTHR
8   ARGGLYALATYRSERSERHISASP

Samples:

sample_1: RCK4, [U-98% 15N], 0.8 – 1.0 mM; sodium phosphate 50 mM; DTT 10 mM; pefablock SC 1 mM

Ex-cond_1: pH: 4.4; temperature: 283 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19426
PDB
DBJ BAC29309
EMBL CAA34133
GB AAA41469 AAB60668 AAI09015 EDL27774 EDL79747
REF NP_037103 NP_067250 XP_003497608 XP_005064870 XP_005364106
SP P15385 Q61423